Overview

  • Product nameAnti-CRF1 antibody
    See all CRF1 primary antibodies
  • Description
    Goat polyclonal to CRF1
  • Tested applicationsSuitable for: IHC-P, ELISA, IHC-FoFr, WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Sheep, Chicken, Human, Pufferfish (Fugu), Rhesus monkey, African Green Monkey, Syrian Hamster
  • Immunogen

    Synthetic peptide corresponding to Human CRF1 aa 279-292 (internal sequence) (Cysteine residue). Synthetic peptide:

    KLYYDNEKCWFGKR

    by a Cysteine residue linker, corresponding to internal sequence amino acids 279-292 of Human CRF1(Peptide available as ab113546.)
    Sequence:

    KLYYDNEKCWFGKR


    (Peptide available as ab113546)

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab59023 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100.
ELISA 1/16000. (Peptide)
IHC-FoFr Use at an assay dependent dilution. PubMed: 20383137
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).Can be blocked with Human CRF1 peptide (ab113546).

Target

  • FunctionThis is a receptor for corticotropin releasing factor. Shows high-affinity CRF binding. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.
  • Tissue specificityPredominantly expressed in the cerebellum, pituitary, cerebral cortex and olfactory lobe.
  • Sequence similaritiesBelongs to the G-protein coupled receptor 2 family.
  • Post-translational
    modifications
    C-terminal Ser or Thr residues may be phosphorylated.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Corticotropin releasing factor type 1 receptor antibody
    • corticotropin releasing hormone receptor 1 antibody
    • Corticotropin-releasing factor receptor 1 antibody
    • Corticotropin-releasing hormone receptor 1 antibody
    • CRF R antibody
    • crf receptor 1 antibody
    • crf receptor type 1 antibody
    • crf type 1 antibody
    • CRF-R antibody
    • CRF-R-1 antibody
    • CRF-R1 antibody
    • CRF1 antibody
    • CRFR antibody
    • CRFR-1 antibody
    • CRFR1 antibody
    • CRFR1_HUMAN antibody
    • CRH-R-1 antibody
    • CRH-R1 antibody
    • CRH-R1h antibody
    • CRHR antibody
    • Crhr1 antibody
    • CRHR1f antibody
    • CRHR1L antibody
    see all

Anti-CRF1 antibody images

  • ab59023 (1/100) staining CRF1 in paraffin embedded mouse brain tissue sections. Tissues were fixed in paraformaldehyde and permeabilised with 0.3% Triton X100/TBS. Please refer to abreview for further experimental details.

    See Abreview

  • All lanes : Anti-CRF1 antibody (ab59023) at 1 µg/ml

    Lane 1 : Rat Forebrain
    Lane 2 : Rat Cortex Tissue Lysate
    Lane 3 : Cerebellum Rat Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 50 kDa
    Observed band size : 50 kDa
    Additional bands at : 110 kDa,70 kDa,75 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes

References for Anti-CRF1 antibody (ab59023)

This product has been referenced in:
  • Johnson AC  et al. Knockdown of corticotropin-releasing factor in the central amygdala reverses persistent viscerosomatic hyperalgesia. Transl Psychiatry 5:e517 (2015). Read more (PubMed: 25734510) »
  • Liang B  et al. Restraint stress impairs oocyte developmental potential in mice: role of CRH-induced apoptosis of ovarian cells. Biol Reprod 89:64 (2013). Read more (PubMed: 23884643) »

See all 4 Publications for this product

Product Wall

Application Western blot
Sample Mouse Tissue lysate - whole (Intestinal tissue)
Gel Running Conditions Reduced Denaturing (4-12% Bis Tris MOPS)
Loading amount 14 µg
Specification Intestinal tissue
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jun 22 2015

Application Western blot
Sample Rat Tissue lysate - whole (Brain)
Loading amount 30 µg
Specification Brain
Gel Running Conditions Reduced Denaturing (Gradient)
Blocking step Casein as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jan 04 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Brain)
Specification Brain
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization Yes - Triton-X
Blocking step Casein as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Oct 15 2012

Thank you for contacting us.

As we discussed on the phone, adding a BSA block and trying a shorter antigen retrieval time may help to reduce the background for ab7963, ab59023, and ab75168, while trying a 1:100 dilution for ab35748 may help t...

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DISCOUNT CODES :
**** for ab1376
**** for ab59023
Expiration date: DD MM YYYY for both codes

I am very pleased to hear you would like to accept our offer and test ab1376 and ab59023 in Pig.Each code will give you 1 freeprimary...

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Thank you for your inquiry.
Unfortunately, this antibody was not tested with samples from a knock out mouse in WB by our lab. We also do not have any data/information about other customers using this antibody in CRF1 ko mice.
I am sorry I did...

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Thank you for contacting us.

I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

I a...

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I am sorry that you are having difficulties using some of our antibodies in western blot. From the data on the website, it does appear that in addition to recognizing the bands of interest, for ab59023 a 50kDa band and for ab75168, a band at 48kDa, bo...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Brain slices)
Specification Brain slices
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization Yes - 0.3% Triton X100
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Dr. Christoph Schwarzer

Verified customer

Submitted Jan 31 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"