Anti-Cripto1 antibody - Embryonic Stem Cell Marker (ab19917)


  • Product nameAnti-Cripto1 antibody - Embryonic Stem Cell Marker
    See all Cripto1 primary antibodies
  • Description
    Rabbit polyclonal to Cripto1 - Embryonic Stem Cell Marker
  • Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Cow, Zebrafish
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human Cripto.

    (Peptide available as ab23310.)

  • Positive control
    • Human Embryonic Stem Cells, Cripto1 Recombinant Protein



Our Abpromise guarantee covers the use of ab19917 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 21 kDa).


  • FunctionCould play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm.
  • Tissue specificityPreferentially expressed in gastric and colorectal carcinomas than in their normal counterparts.
  • Sequence similaritiesContains 1 EGF-like domain.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CR antibody
    • CRGF antibody
    • Cripto 1 antibody
    • Cripto 1 growth factor antibody
    • cripto antibody
    • Cripto-1 growth factor antibody
    • Cripto1 growth factor antibody
    • Epidermal growth factor like cripto protein CR1 antibody
    • Epidermal growth factor-like cripto protein CR1 antibody
    • TDGF 1 antibody
    • TDGF1 antibody
    • TDGF1_HUMAN antibody
    • Teratocarcinoma derived growth factor 1 antibody
    • Teratocarcinoma-derived growth factor 1 antibody
    see all

Anti-Cripto1 antibody - Embryonic Stem Cell Marker images

  • ICC/IF image of ab19917 stained mouse embryonic stem cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab19917, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • Anti-Cripto antibody, ab19917, stained the membrane of human embryonic stem cells at a dilution of 1/100.
  • The image shows staining of human pancreas tissue. Staining was nuclear in some islet cells and cytoplasmic in some cells of the acini. Antigen retrieval was performed using Citrate at pH6. Nuclear localization of Cripto protein has been described in the literature: Gong YP et al. Eur J Surg Oncol, 2006.

References for Anti-Cripto1 antibody - Embryonic Stem Cell Marker (ab19917)

This product has been referenced in:
  • Spike BT  et al. CRIPTO/GRP78 Signaling Maintains Fetal and Adult Mammary Stem Cells Ex Vivo. Stem Cell Reports 2:427-39 (2014). ICC/IF ; Human . Read more (PubMed: 24749068) »
  • Spiller CM  et al. Endogenous Nodal signaling regulates germ cell potency during mammalian testis development. Development 139:4123-32 (2012). IHC-P ; Mouse . Read more (PubMed: 23034635) »

See all 5 Publications for this product

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I am sorry we do not sell the human embryonic stem cells.

As far as we are aware, this antibody has not been tested in other sample types. I can recommend the customer try a literature search to obtain ...

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