Overview

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab101032 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 27 kDa). Preliminary experiments gave an approx 55kDa band in Human Testis lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 27.3kDa according to NP_003287.1. The 55kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field. Have any further splice variants/modified forms been reported?

Target

  • Function
    May regulate some ion channels' activity and therebye regulate calcium fluxes during sperm capacitation.
  • Tissue specificity
    Testis and epididymis.
  • Sequence similarities
    Belongs to the CRISP family.
    Contains 1 SCP domain.
    Contains 1 ShKT domain.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cancer/testis antigen 36 antibody
    • CRIS2_HUMAN antibody
    • CRISP 2 antibody
    • CRISP-2 antibody
    • CRISP2 antibody
    • CT36 antibody
    • Cysteine rich secretory protein 2 antibody
    • Cysteine-rich secretory protein 2 antibody
    • GAPDL5 antibody
    • Glyceraldehyde 3 phosphate dehydrogenase like 5 antibody
    • Testis specific protein 1 (probe H4 1 p3 1) antibody
    • Testis specific protein TPX1 antibody
    • Testis-specific protein TPX-1 antibody
    • TPX1 antibody
    • TSP1 antibody
    see all

Images

  • Lane 1 : Anti-CRISP2 antibody (ab101032) at 1 µg/ml
    Lanes 2 - 3 : Anti-MYC tag at 1/1000 dilution

    Lane 1 : HEK293 overexpressing CRISP2 with MYC tag cell lysate in RIPA buffer
    Lane 2 : Mock-transfected HEK293 cell lysate in RIPA buffer
    Lane 3 : HEK293 overexpressing CRISP2 with MYC tag cell lysate in RIPA buffer

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 27 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)

References

ab101032 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab101032.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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