Overview

  • Product nameAnti-CRISP3 antibody [LV-2A2]
    See all CRISP3 primary antibodies
  • Description
    Mouse monoclonal [LV-2A2] to CRISP3
  • Tested applicationsSuitable for: Flow Cyt, Competitive ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Genetic immunisation with cDNA encoding human CRISP3

  • Positive control
    • BOSC23 cells transfected with an expression vector encoding human CRISP3.
  • General notes


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. Genetic immunization was with cDNA encoding human CRISP3 and selection was based on recognition of the complete native protein expressed on transfected mammalian cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • Primary antibody notesThis antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. Genetic immunization was with cDNA encoding human CRISP3 and selection was based on recognition of the complete native protein expressed on transfected mammalian cells.
  • ClonalityMonoclonal
  • Clone numberLV-2A2
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab62225 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1.2µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Competitive ELISA 1/200 - 1/400.

Target

  • Tissue specificitySalivary gland, pancreas and prostate > epididymis, ovary, thymus and colon.
  • Sequence similaritiesBelongs to the CRISP family.
  • Cellular localizationSecreted. In neutrophils, localized in specific granules.
  • Information by UniProt
  • Database links
  • Alternative names
    • Aeg 2 antibody
    • Aeg2 antibody
    • CRIS3_HUMAN antibody
    • CRISP 3 antibody
    • CRISP-3 antibody
    • Crisp3 antibody
    • CRS 3 antibody
    • CRS3 antibody
    • Cysteine rich secretory protein 3 antibody
    • Cysteine-rich secretory protein 3 antibody
    • dJ442L6.3 antibody
    • MGC126588 antibody
    • OTTHUMP00000016590 antibody
    • OTTHUMP00000039911 antibody
    • SGP 28 antibody
    • SGP28 antibody
    • SGP28 protein antibody
    • Specific granule protein (28 kDa) antibody
    • Specific granule protein of 28 kDa antibody
    see all

Anti-CRISP3 antibody [LV-2A2] images

  • FACS analysis of BOSC23 cells using CRISP3 antibody [LV-2A2] (ab62225). BOSC23 cells were transiently transfected with an expression vector encoding either CRISP3 (red curve) or an irrelevant protein (control transfectant: black curve). Binding of ab62225 was detected with a PE conjugated secondary antibody. A positive signal was obtained only with CRISP3 transfected cells.

References for Anti-CRISP3 antibody [LV-2A2] (ab62225)

ab62225 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"