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Genetic immunisation with cDNA encoding human CRISP3
This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. Genetic immunization was with cDNA encoding human CRISP3 and selection was based on recognition of the complete native protein expressed on transfected mammalian cells.
Our Abpromise guarantee covers the use of ab62225 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1.2µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
|Competitive ELISA||1/200 - 1/400.|
ab62225 has not yet been referenced specifically in any publications.