Overview

  • Product nameAnti-Crk p38 antibody [2210C1a]
    See all Crk p38 primary antibodies
  • Description
    Mouse monoclonal [2210C1a] to Crk p38
  • Tested applicationsSuitable for: WB, Dot blotmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant human CRK

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage bufferPreservative: 0.05% Sodium Azide
    Constituents: 1% BSA, PBS (8mM Sodium phosphate, 3mM Potassium chloride, 140mM Sodium chloride, 1.5mM Potassium phosphate, pH 7.4).
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesAb51347 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG depleted (approximately 95%) fetal bovine serum.
  • ClonalityMonoclonal
  • Clone number2210C1a
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab51347 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Predicted molecular weight: 34 kDa.
Dot blot Use at an assay dependent dilution.

Target

  • FunctionThe Crk-I and Crk-II forms differ in their biological activities. Crk-II has less transforming activity than Crk-I. Crk-II mediates attachment-induced MAPK8 activation, membrane ruffling and cell motility in a Rac-dependent manner. Involved in phagocytosis of apoptotic cells and cell motility via its interaction with DOCK1 and DOCK4.
  • Sequence similaritiesBelongs to the CRK family.
    Contains 1 SH2 domain.
    Contains 2 SH3 domains.
  • DomainThe C-terminal SH3 domain function as a negative modulator for transformation and the N-terminal SH3 domain appears to function as a positive regulator for transformation.
    The SH2 domain mediates interaction with SHB.
  • Post-translational
    modifications
    Phosphorylation of Crk-II (40 kDa) gives rise to a 42 kDa form.
    Phosphorylated on Tyr-221 upon cell adhesion. Results in the negative regulation of the association with SH2- and SH3-binding partners, possibly by the formation of an intramolecular interaction of phosphorylated Tyr-221 with the SH2 domain. This leads finally to the down-regulation of the Crk signaling pathway.
  • Cellular localizationCytoplasm. Cell membrane. Translocated to the plasma membrane upon cell adhesion.
  • Information by UniProt
  • Database links
  • Alternative names
    • Adapter molecule crk antibody
    • Avian sarcoma virus CT10 (v crk) oncogene homolog antibody
    • CRK antibody
    • CRK isoform 2 antibody
    • CRK isoform II antibody
    • CRK_HUMAN antibody
    • CRKII antibody
    • FLJ38130 antibody
    • OTTHUMP00000115366 antibody
    • OTTHUMP00000198330 antibody
    • p38 antibody
    • Proto oncogene C crk antibody
    • Proto-oncogene C-crk antibody
    • v crk avian sarcoma virus CT10 oncogene homolog antibody
    • v crk sarcoma virus CT10 oncogene homolog antibody
    • v crk sarcoma virus CT10 oncogene homolog (avian) antibody
    see all

Anti-Crk p38 antibody [2210C1a] images

References for Anti-Crk p38 antibody [2210C1a] (ab51347)

ab51347 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"