• Product name
    Anti-Crk p38 antibody [EP242Y]
    See all Crk p38 primary antibodies
  • Description
    Rabbit monoclonal [EP242Y] to Crk p38
  • Tested applications
    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in the second SH3 domain and near C-terminus of human Crk p38 protein.

  • Positive control
    • HepG2 cell lysate (WB) and HeLa cells (ICC/IF).
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.



Our Abpromise guarantee covers the use of ab45136 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notes
    ICC/IF: 1/250 - 1/500.
    IP: 1/50.
    WB: 1/10000. Detects a band of approximately 37 kDa (predicted molecular weight: 34 kDa).

    Is unsuitable for Flow Cyt or IHC.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      The Crk-I and Crk-II forms differ in their biological activities. Crk-II has less transforming activity than Crk-I. Crk-II mediates attachment-induced MAPK8 activation, membrane ruffling and cell motility in a Rac-dependent manner. Involved in phagocytosis of apoptotic cells and cell motility via its interaction with DOCK1 and DOCK4.
    • Sequence similarities
      Belongs to the CRK family.
      Contains 1 SH2 domain.
      Contains 2 SH3 domains.
    • Domain
      The C-terminal SH3 domain function as a negative modulator for transformation and the N-terminal SH3 domain appears to function as a positive regulator for transformation.
      The SH2 domain mediates interaction with SHB.
    • Post-translational
      Phosphorylation of Crk-II (40 kDa) gives rise to a 42 kDa form.
      Phosphorylated on Tyr-221 upon cell adhesion. Results in the negative regulation of the association with SH2- and SH3-binding partners, possibly by the formation of an intramolecular interaction of phosphorylated Tyr-221 with the SH2 domain. This leads finally to the down-regulation of the Crk signaling pathway.
    • Cellular localization
      Cytoplasm. Cell membrane. Translocated to the plasma membrane upon cell adhesion.
    • Information by UniProt
    • Database links
    • Alternative names
      • Adapter molecule crk antibody
      • Avian sarcoma virus CT10 (v crk) oncogene homolog antibody
      • CRK antibody
      • CRK isoform 2 antibody
      • CRK isoform II antibody
      • CRK_HUMAN antibody
      • CRKII antibody
      • FLJ38130 antibody
      • OTTHUMP00000115366 antibody
      • OTTHUMP00000198330 antibody
      • p38 antibody
      • Proto oncogene C crk antibody
      • Proto-oncogene C-crk antibody
      • v crk avian sarcoma virus CT10 oncogene homolog antibody
      • v crk sarcoma virus CT10 oncogene homolog antibody
      • v crk sarcoma virus CT10 oncogene homolog (avian) antibody
      see all

    Anti-Crk p38 antibody [EP242Y] images

    • Anti-Crk p38 antibody [EP242Y] (ab45136) at 1/10000 dilution + HepG2 cell lysate at 10 µg

      Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 34 kDa
      Observed band size : 37 kDa (why is the actual band size different from the predicted?)
    • Immunofluorescent staining of HeLa cells using ab45136 at a 1/250 dilution.

    References for Anti-Crk p38 antibody [EP242Y] (ab45136)

    This product has been referenced in:
    • Ahn J  et al. The metastasis gene NEDD9 product acts through integrin ß3 and Src to promote mesenchymal motility and inhibit amoeboid motility. J Cell Sci 125:1814-26 (2012). WB . Read more (PubMed: 22328516) »

    See 1 Publication for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Western blot
    Mouse Tissue lysate - whole (mouse brain)
    Loading amount
    10 µg
    mouse brain
    Gel Running Conditions
    Reduced Denaturing (12% SDS-PAGE)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted May 18 2011


    Sign up