Anti-Crk p38 (phospho Y221) antibody (ab51222)

Overview

  • Product name
    Anti-Crk p38 (phospho Y221) antibody
    See all Crk p38 primary antibodies
  • Description
    Rabbit polyclonal to Crk p38 (phospho Y221)
  • Specificity
    This antibody detects endogenous levels of CRK isoform II only when phosphorylated at tyrosine 221.
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    A synthesized phosphopeptide derived from human CRK isoform II around the phosphorylation site of tyrosine 221 (G-P-YP-A-Q).

  • Positive control
    • Extracts of COS7 cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab51222 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/300 - 1/1000. Detects a band of approximately 44 kDa (predicted molecular weight: 34 kDa).
ELISA 1/40000.

Target

  • Function
    The Crk-I and Crk-II forms differ in their biological activities. Crk-II has less transforming activity than Crk-I. Crk-II mediates attachment-induced MAPK8 activation, membrane ruffling and cell motility in a Rac-dependent manner. Involved in phagocytosis of apoptotic cells and cell motility via its interaction with DOCK1 and DOCK4.
  • Sequence similarities
    Belongs to the CRK family.
    Contains 1 SH2 domain.
    Contains 2 SH3 domains.
  • Domain
    The C-terminal SH3 domain function as a negative modulator for transformation and the N-terminal SH3 domain appears to function as a positive regulator for transformation.
    The SH2 domain mediates interaction with SHB.
  • Post-translational
    modifications
    Phosphorylation of Crk-II (40 kDa) gives rise to a 42 kDa form.
    Phosphorylated on Tyr-221 upon cell adhesion. Results in the negative regulation of the association with SH2- and SH3-binding partners, possibly by the formation of an intramolecular interaction of phosphorylated Tyr-221 with the SH2 domain. This leads finally to the down-regulation of the Crk signaling pathway.
  • Cellular localization
    Cytoplasm. Cell membrane. Translocated to the plasma membrane upon cell adhesion.
  • Information by UniProt
  • Database links
  • Alternative names
    • Adapter molecule crk antibody
    • Avian sarcoma virus CT10 (v crk) oncogene homolog antibody
    • CRK antibody
    • CRK isoform 2 antibody
    • CRK isoform II antibody
    • CRK_HUMAN antibody
    • CRKII antibody
    • FLJ38130 antibody
    • OTTHUMP00000115366 antibody
    • OTTHUMP00000198330 antibody
    • p38 antibody
    • Proto oncogene C crk antibody
    • Proto-oncogene C-crk antibody
    • v crk avian sarcoma virus CT10 oncogene homolog antibody
    • v crk sarcoma virus CT10 oncogene homolog antibody
    • v crk sarcoma virus CT10 oncogene homolog (avian) antibody
    see all

Anti-Crk p38 (phospho Y221) antibody images

  • All lanes : Anti-Crk p38 (phospho Y221) antibody (ab51222) at 1/300 dilution

    Lane 1 : Extracts of cos7 cells treated with phosphopeptide
    Lane 2 : Extracts of cos7 cells


    Predicted band size : 34 kDa
    Observed band size : 44 kDa (why is the actual band size different from the predicted?)

References for Anti-Crk p38 (phospho Y221) antibody (ab51222)

ab51222 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Mammary epithelial cells(MCF7))
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
40 µg
Specification
Mammary epithelial cells(MCF7)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Username

Abcam user community

Verified customer

Submitted Sep 22 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (mammary epithelial cells(mcf7))
Total protein in input
40 µg
Immuno-precipitation step
Protein A/G
Specification
mammary epithelial cells(mcf7)
Username

Abcam user community

Verified customer

Submitted Sep 20 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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