Recombinant
RabMAb

Anti-Crk p38 (phospho Y221) antibody [EPR269Y] (ab76227)

Overview

  • Product name
    Anti-Crk p38 (phospho Y221) antibody [EPR269Y]
    See all Crk p38 primary antibodies
  • Description
    Rabbit monoclonal [EPR269Y] to Crk p38 (phospho Y221)
  • Specificity
    This antibody detects proteins phosphorylated at tyrosine 221.
  • Tested applications
    Suitable for: WB, ICCmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic phosphopeptide corresponding to regions surrounding Tyrosine 221 of human Crk p38

  • Positive control
    • K562 cell lysate
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR269Y
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab76227 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Predicted molecular weight: 34 kDa.
ICC 1/50 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Function
      The Crk-I and Crk-II forms differ in their biological activities. Crk-II has less transforming activity than Crk-I. Crk-II mediates attachment-induced MAPK8 activation, membrane ruffling and cell motility in a Rac-dependent manner. Involved in phagocytosis of apoptotic cells and cell motility via its interaction with DOCK1 and DOCK4.
    • Sequence similarities
      Belongs to the CRK family.
      Contains 1 SH2 domain.
      Contains 2 SH3 domains.
    • Domain
      The C-terminal SH3 domain function as a negative modulator for transformation and the N-terminal SH3 domain appears to function as a positive regulator for transformation.
      The SH2 domain mediates interaction with SHB.
    • Post-translational
      modifications
      Phosphorylation of Crk-II (40 kDa) gives rise to a 42 kDa form.
      Phosphorylated on Tyr-221 upon cell adhesion. Results in the negative regulation of the association with SH2- and SH3-binding partners, possibly by the formation of an intramolecular interaction of phosphorylated Tyr-221 with the SH2 domain. This leads finally to the down-regulation of the Crk signaling pathway.
    • Cellular localization
      Cytoplasm. Cell membrane. Translocated to the plasma membrane upon cell adhesion.
    • Information by UniProt
    • Database links
    • Alternative names
      • Adapter molecule crk antibody
      • Avian sarcoma virus CT10 (v crk) oncogene homolog antibody
      • CRK antibody
      • CRK isoform 2 antibody
      • CRK isoform II antibody
      • CRK_HUMAN antibody
      • CRKII antibody
      • FLJ38130 antibody
      • OTTHUMP00000115366 antibody
      • OTTHUMP00000198330 antibody
      • p38 antibody
      • Proto oncogene C crk antibody
      • Proto-oncogene C-crk antibody
      • v crk avian sarcoma virus CT10 oncogene homolog antibody
      • v crk sarcoma virus CT10 oncogene homolog antibody
      • v crk sarcoma virus CT10 oncogene homolog (avian) antibody
      see all

    Images

    • All lanes : Anti-Crk p38 (phospho Y221) antibody [EPR269Y] (ab76227) at 1/2000 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : K562 cell lysate treated with AP

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 34 kDa
      Observed band size : 34 kDa

    References

    This product has been referenced in:
    • Nieto-Pelegrin E  et al. Crk adaptors negatively regulate actin polymerization in pedestals formed by enteropathogenic Escherichia coli (EPEC) by binding to Tir effector. PLoS Pathog 10:e1004022 (2014). WB ; Human . Read more (PubMed: 24675776) »
    • Li XN  et al. The p38 MAPK inhibitor JLU1124 inhibits the inflammatory response induced by lipopolysaccharide through the MAPK-NF-?B pathway in RAW264.7 macrophages. Int Immunopharmacol 17:785-92 (2013). Read more (PubMed: 24070708) »

    See all 4 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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