Application notesIs unsuitable for Flow Cyt,IHC-P or IP.
FunctionThe Crk-I and Crk-II forms differ in their biological activities. Crk-II has less transforming activity than Crk-I. Crk-II mediates attachment-induced MAPK8 activation, membrane ruffling and cell motility in a Rac-dependent manner. Involved in phagocytosis of apoptotic cells and cell motility via its interaction with DOCK1 and DOCK4.
Sequence similaritiesBelongs to the CRK family. Contains 1 SH2 domain. Contains 2 SH3 domains.
DomainThe C-terminal SH3 domain function as a negative modulator for transformation and the N-terminal SH3 domain appears to function as a positive regulator for transformation. The SH2 domain mediates interaction with SHB.
Post-translational modificationsPhosphorylation of Crk-II (40 kDa) gives rise to a 42 kDa form. Phosphorylated on Tyr-221 upon cell adhesion. Results in the negative regulation of the association with SH2- and SH3-binding partners, possibly by the formation of an intramolecular interaction of phosphorylated Tyr-221 with the SH2 domain. This leads finally to the down-regulation of the Crk signaling pathway.
Cellular localizationCytoplasm. Cell membrane. Translocated to the plasma membrane upon cell adhesion.
Western blot - Crk p38 (phospho Y221) antibody [EPR269Y] (ab76227)
All lanes : Anti-Crk p38 (phospho Y221) antibody [EPR269Y] (ab76227) at 1/2000 dilution
Lane 1 : K562 cell lysate Lane 2 : K562 cell lysate treated with AP
Lysates/proteins at 10 µg per lane.
Secondary HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size : 34 kDa Observed band size : 34 kDa
References for Anti-Crk p38 (phospho Y221) antibody [EPR269Y] (ab76227)
This product has been referenced in:
Nieto-Pelegrin E et al. Crk adaptors negatively regulate actin polymerization in pedestals formed by enteropathogenic Escherichia coli (EPEC) by binding to Tir effector. PLoS Pathog10:e1004022 (2014).
Read more (PubMed: 24675776) »
Li XN et al. The p38 MAPK inhibitor JLU1124 inhibits the inflammatory response induced by lipopolysaccharide through the MAPK-NF-?B pathway in RAW264.7 macrophages. Int Immunopharmacol17:785-92 (2013).
Read more (PubMed: 24070708) »