Overview

  • Product name
  • Description
    Goat polyclonal to CrkL
  • Host species
    Goat
  • Tested applications
    Suitable for: WB, ICC, ELISA, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide:

    KIFDPQNPDENE

    , corresponding to C terminal amino acids 292-303 of Human CrkL

Properties

Applications

Our Abpromise guarantee covers the use of ab1302 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.01 - 0.03 µg/ml. Predicted molecular weight: 33 kDa.
ICC Use a concentration of 2.5 - 3.8 µg/ml.
ELISA Use at an assay dependent dilution.
IP Use at an assay dependent concentration.

Target

Images

  • Anti-CrkL antibody (ab1302) at 0.01 µg/ml + K562 lysate (35µg protein in RIPA buffer).

    Predicted band size: 33 kDa



    Primary incubation was 1 hour. Detected by chemiluminescence.
  • ab1302 (3.8µg/ml) staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

References

ab1302 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab1302.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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