Overview

  • Product nameAnti-CrkL antibody
    See all CrkL primary antibodies
  • Description
    Rabbit polyclonal to CrkL
  • Tested applicationsSuitable for: WB, IHC-P, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic non-phosphopeptide derived from human CrkL around the phosphorylation site of tyrosine 207 (H-A-YP-A-Q).

  • Positive control
    • Human lung carcinoma tissue, extracts from 293 cells, HuvEc cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab63491 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/300 - 1/1000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
IHC-P 1/50 - 1/100.
ELISA 1/10000.
ICC/IF 1/500 - 1/1000.

Target

Anti-CrkL antibody images

  • This image shows HuvEc cells stained with ab63491 at 1/500 dilution; with and without the immunising peptide.
  • This image shows human lung carcinoma tissue stained with ab63491 at 1/50 dilution; with and without the immunising peptide.
  • All lanes : Anti-CrkL antibody (ab63491) at 1/300 dilution

    Lane 1 : Extracts from 293 cells (5-30µg total protein)
    Lane 2 : Extracts from 293 cells (5-30µg total protein) and the immunising peptide (5-10µg).


    Predicted band size : 34 kDa
    Observed band size : 34 kDa

References for Anti-CrkL antibody (ab63491)

ab63491 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"