Overview

  • Product name
    Anti-CrkL antibody [Y243]
    See all CrkL primary antibodies
  • Description
    Rabbit monoclonal [Y243] to CrkL
  • Tested applications
    Suitable for: WB, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues in the SH2 domain, near the N-term of human CrkL

  • Positive control
    • K562 cell lysate and human breast carcinoma
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab32126 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 33 kDa).
IHC-P 1/50 - 1/100.
ICC 1/50 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function
      May mediate the transduction of intracellular signals.
    • Sequence similarities
      Belongs to the CRK family.
      Contains 1 SH2 domain.
      Contains 2 SH3 domains.
    • Information by UniProt
    • Database links
    • Alternative names
      • Crk L antibody
      • Crk like protein antibody
      • Crk-like protein antibody
      • Crkl antibody
      • CRKL_HUMAN antibody
      • Crkol antibody
      • HGNC:2363 antibody
      • Oncogene CrkL antibody
      • V crk avian sarcoma virus CT10 oncogene homolog like antibody
      • v crk sarcoma virus CT10 oncogene homolog (avian) like antibody
      • V crk sarcoma virus CT10 oncogene homolog avian like antibody
      see all

    Anti-CrkL antibody [Y243] images

    • Anti-CrkL antibody [Y243] (ab32126) at 1/1000 dilution + K562 cell lysate

      Predicted band size : 33 kDa
      Observed band size : 39 kDa (why is the actual band size different from the predicted?)
    • ab32126, staining human breast carcinoma by immunohistochemistry, Paraffin embedded tissue

    References for Anti-CrkL antibody [Y243] (ab32126)

    This product has been referenced in:
    • Poulsen ET  et al. New Insights to Clathrin and Adaptor Protein 2 for the Design and Development of Therapeutic Strategies. Int J Mol Sci 16:29446-53 (2015). IP . Read more (PubMed: 26690411) »
    • Wang J  et al. SCG10 promotes non-amyloidogenic processing of amyloid precursor protein by facilitating its trafficking to the cell surface. Hum Mol Genet N/A:N/A (2013). Read more (PubMed: 23863461) »

    See all 3 Publications for this product

    Product Wall

    Thank you for your inquiry. I have confirmed that neither of these two anti-CrkL antibodies, ab32018 or ab32126, bind within the region a.a. 123 - 183 (the first SH3 domain). I apologize but it doesn't look like any of the anti-CrkL ant...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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