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Rabbit monoclonal [EP270Y] to CrkL (phospho Y207)
ab52908 only detects CrkL phosphorylated on Tyrosine 207.
IP more details Unsuitable for:
Flow Cyt or IHC-P
A synthetic phospho peptide corresponding to residues surrounding Tyr207 of human CrkL
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb
® technology. RabMAb ® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Tissue culture supernatant
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50000. Detects a band of approximately 39 kDa (predicted molecular weight: 34 kDa).
Is unsuitable for Flow Cyt or IHC-P.
Anti-CrkL (phospho Y207) antibody [EP270Y] images
Western blot - CrkL antibody [EP270Y] (ab52908)
All lanes :
Anti-CrkL (phospho Y207) antibody [EP270Y] (ab52908) at 1/50000 dilution
Lane 1 :
(A) K562 cell lysates untreated
Lane 2 :
(B) K562 cell lysates treated with AP
Lysates/proteins at 10 µg per lane.
Predicted band size :
Observed band size :
37 kDa (
why is the actual band size different from the predicted?
References for Anti-CrkL (phospho Y207) antibody [EP270Y] (ab52908)
This product has been referenced in:
et al. Inhibition of 32Dp210 cells harboring T315I mutation by a novel derivative of emodin correlates with down-regulation of BCR-ABL and its downstream signaling pathways. J Cancer Res Clin Oncol N/A:N/A (2014).
Read more (PubMed: 25217883) »
et al. A multiple reaction monitoring (MRM) method to detect Bcr-Abl kinase activity in CML using a peptide biosensor. PLoS One 8:e56627 (2013).
Read more (PubMed: 23437189) »
See all 4 Publications
for this product
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"