Overview

  • Product nameAnti-CrkL (phospho Y207) antibody [EP270Y]
    See all CrkL primary antibodies
  • Description
    Rabbit monoclonal [EP270Y] to CrkL (phospho Y207)
  • Specificityab52908 only detects CrkL phosphorylated on Tyrosine 207.
  • Tested applicationsSuitable for: WB, IPmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A synthetic phospho peptide corresponding to residues surrounding Tyr207 of human CrkL

  • Positive control
    • WB: K562 cell lysates
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab52908 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50000. Detects a band of approximately 39 kDa (predicted molecular weight: 34 kDa).
IP 1/50.
  • Application notesIs unsuitable for Flow Cyt or IHC-P.
  • Target

    Anti-CrkL (phospho Y207) antibody [EP270Y] images

    • All lanes : Anti-CrkL (phospho Y207) antibody [EP270Y] (ab52908) at 1/50000 dilution

      Lane 1 : (A) K562 cell lysates untreated
      Lane 2 : (B) K562 cell lysates treated with AP

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 34 kDa
      Observed band size : 37 kDa (why is the actual band size different from the predicted?)

    References for Anti-CrkL (phospho Y207) antibody [EP270Y] (ab52908)

    This product has been referenced in:
    • Li J  et al. Inhibition of 32Dp210 cells harboring T315I mutation by a novel derivative of emodin correlates with down-regulation of BCR-ABL and its downstream signaling pathways. J Cancer Res Clin Oncol N/A:N/A (2014). Human . Read more (PubMed: 25217883) »
    • Yang TY  et al. A multiple reaction monitoring (MRM) method to detect Bcr-Abl kinase activity in CML using a peptide biosensor. PLoS One 8:e56627 (2013). WB ; Human . Read more (PubMed: 23437189) »

    See all 4 Publications for this product

    Product Wall

    There are currently no Abreviews or Questions for ab52908.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"