Recombinant
RabMAb

Anti-CrkL (phospho Y207) antibody [EP270Y] (ab52908)

Overview

  • Product name
    Anti-CrkL (phospho Y207) antibody [EP270Y]
    See all CrkL primary antibodies
  • Description
    Rabbit monoclonal [EP270Y] to CrkL (phospho Y207)
  • Specificity
    ab52908 only detects CrkL phosphorylated on Tyrosine 207.
  • Tested applications
    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A synthetic phospho peptide corresponding to residues surrounding Tyr207 of human CrkL

  • Positive control
    • WB: K562 cell lysates
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab52908 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50000. Detects a band of approximately 39 kDa (predicted molecular weight: 34 kDa).
IP 1/50.
  • Application notes
    Is unsuitable for Flow Cyt or IHC-P.
  • Target

    Images

    • All lanes : Anti-CrkL (phospho Y207) antibody [EP270Y] (ab52908) at 1/50000 dilution

      Lane 1 : (A) K562 cell lysates untreated
      Lane 2 : (B) K562 cell lysates treated with AP

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 34 kDa
      Observed band size : 37 kDa (why is the actual band size different from the predicted?)

    References

    This product has been referenced in:
    • Ouellette SB  et al. A Cell-Based Assay for Measuring Endogenous BcrAbl Kinase Activity and Inhibitor Resistance. PLoS One 11:e0161748 (2016). Read more (PubMed: 27598410) »
    • Li J  et al. Inhibition of 32Dp210 cells harboring T315I mutation by a novel derivative of emodin correlates with down-regulation of BCR-ABL and its downstream signaling pathways. J Cancer Res Clin Oncol N/A:N/A (2014). Human . Read more (PubMed: 25217883) »

    See all 6 Publications for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab52908.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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