Mouse, Rat, Human Predicted to work with:
Rabbit, Chicken, Pig, Chimpanzee, Zebrafish, Macaque monkey, Gorilla, Chinese hamster, Orangutan
Synthetic peptide corresponding to Human CRLS1 aa 150-250 conjugated to Keyhole Limpet Haemocyanin (KLH). Database link: Q9UJA2
This antibody gave a positive signal in the following tissue lysates: Human Heart; Mouse Heart; Rat Heart; Human Skeletal Muscle; Mouse Skeletal Muscle; Human Liver; Rat Liver.
It also gave a positive result in IHC-P in FFPE human heart tissue sections.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
IHC image of CRLS1 staining in human heart formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab156882, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Western blot - Anti-CRLS1 antibody (ab156882)
All lanes : Anti-CRLS1 antibody (ab156882) at 1 µg/ml
Lane 1 : Human heart tissue lysate - total protein (ab29431) Lane 2 : Heart (Mouse) Tissue Lysate Lane 3 : Heart (Rat) Tissue Lysate Lane 4 : Human skeletal muscle tissue lysate - total protein (ab29330) Lane 5 : Skeletal Muscle (Mouse) Tissue Lysate Lane 6 : Human liver tissue lysate - total protein (ab29889) Lane 7 : Liver (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab156882 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.