Overview

  • Product name
  • Description
    Rabbit polyclonal to CRM1
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region within C terminal amino acids 1025-1071 (FAGEDTSDLF LEEREIALRQ ADEEKHKRQM SVPGIFNPHE IPEEMCD) of human CRM1 (NP_003391.1).

  • Positive control
    • Human breast tumor; mouse squamous cell carcinoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab84375 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
  • Application notes
    IHC-P: 1/100 - 1/500.
    Epitope exposure is recommended.
    Epitope exposure with citrate buffer will enhance staining.

    Likely to work with frozen sections.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Mediates the nuclear export of cellular proteins (cargos) bearing a leucine-rich nuclear export signal (NES) and of RNAs. In the nucleus, in association with RANBP3, binds cooperatively to the NES on its target protein and to the GTPase RAN in its active GTP-bound form (Ran-GTP). Docking of this complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of an nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the cargo from the export receptor. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Involved in U3 snoRNA transport from Cajal bodies to nucleoli. Binds to late precursor U3 snoRNA bearing a TMG cap. Several viruses, among them HIV-1, HTLV-1 and influenza A use it to export their unspliced or incompletely spliced RNAs out of the nucleus. Interacts with, and mediates the nuclear export of HIV-1 Rev and HTLV-1 Rex proteins. Involved in HTLV-1 Rex multimerization.
    • Tissue specificity
      Expressed in heart, brain, placenta, lung, liver, skeletal muscle, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes. Not expressed in the kidney.
    • Sequence similarities
      Belongs to the exportin family.
      Contains 10 HEAT repeats.
      Contains 1 importin N-terminal domain.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization
      Cytoplasm. Nucleus > nucleoplasm. Nucleus > Cajal body. Nucleus > nucleolus. Located in the nucleoplasm, Cajal bodies and nucleoli. Shuttles between the nucleus/nucleolus and the cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • Chromosome region maintenance 1 protein homolog antibody
      • CRM 1 antibody
      • CRM1 homolog antibody
      • DKFZp686B1823 antibody
      • emb antibody
      • Exp 1 antibody
      • Exp1 antibody
      • Exportin 1 antibody
      • Exportin-1 antibody
      • Exportin1 antibody
      • XPO 1 antibody
      • xpo1 antibody
      • XPO1_HUMAN antibody
      see all

    Images

    • ab84375, at a 1/250 dilution, staining CRM1 in formalin fixed, paraffin embedded human breast tumor section by immunohistochemistry.
      Detection: DAB staining
    • ab84375, at a 1/250 dilution, staining CRM1 in formalin fixed, paraffin embedded mouse squamous cell carcinoma by immunohistochemistry. Detection: DAB staining

    References

    This product has been referenced in:
    • Heerma van Voss MR  et al. Nuclear DDX3 expression predicts poor outcome in colorectal and breast cancer. Onco Targets Ther 10:3501-3513 (2017). Read more (PubMed: 28761359) »

    See 1 Publication for this product

    Customer reviews and Q&As

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