Overview

  • Product name
  • Description
    Rabbit polyclonal to CRMP1
  • Specificity
    No cross reactivity was detected with CRMP2, CRMP3, CRMP4 or CRMP5.
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Fr, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A 15 residue synthetic peptide derived from C-terminal domain of human collapsing-response mediator.

Properties

Applications

Our Abpromise guarantee covers the use of ab36199 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/1000.
WB 1/2000. Detects a band of approximately 65 kDa (predicted molecular weight: 62 kDa).Can be blocked with CRMP1 peptide (ab196857).
IHC-Fr 1/500.
IHC-P Use at an assay dependent concentration.

Target

  • Function
    Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, invasive growth and cell migration.
  • Tissue specificity
    Brain.
  • Sequence similarities
    Belongs to the DHOase family. Hydantoinase/dihydropyrimidinase subfamily.
  • Cellular localization
    Cytoplasm. Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle. Associated with centrosomes and the mitotic spindle during metaphase.
  • Information by UniProt
  • Database links
  • Alternative names
    • Collapsin response mediator protein 1 antibody
    • CRMP 1 antibody
    • CRMP-1 antibody
    • Crmp1 antibody
    • Dihydropyrimidinase like 1 antibody
    • Dihydropyrimidinase related protein 1 antibody
    • Dihydropyrimidinase-related protein 1 antibody
    • DPYL1_HUMAN antibody
    • DPYSL1 antibody
    • DRP 1 antibody
    • DRP-1 antibody
    • DRP1 antibody
    • ULIP-3 antibody
    • Ulip3 antibody
    • Unc-33-like phosphoprotein 3 antibody
    see all

Images

  • All lanes : Anti-CRMP1 antibody (ab36199)

    Lane 1 : E.coli lysate transformed with cDNA of human CRMP1.
    Lane 2 : E.coli lysate transformed with cDNA of human CRMP2.
    Lane 3 : E.coli lysate transformed with cDNA of human CRMP3.
    Lane 4 : E.coli lysate transformed with cDNA of human CRMP4.
    Lane 5 : E.coli lysate transformed with cDNA of human CRMP5.


    Predicted band size : 62 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)
  • Ab36199 staining human CRMP1 in olfactory receptor neurons within the olfactory mucosa by immunohistochemistry.
  • ICC/IF image of ab36199 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab36199, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

ab36199 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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