Overview

  • Product name
  • Description
    Rabbit polyclonal to CRMP1
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A 15 amino acid peptide from near the carboxy terminus of human CRMP1

  • Positive control
    • 3T3 cell lysate. Human brain tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab62558 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 62 kDa).
IHC-P Use a concentration of 2 µg/ml.
ICC/IF Use at an assay dependent concentration. PubMed: 20489728

Target

  • Function
    Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, invasive growth and cell migration.
  • Tissue specificity
    Brain.
  • Sequence similarities
    Belongs to the DHOase family. Hydantoinase/dihydropyrimidinase subfamily.
  • Cellular localization
    Cytoplasm. Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle. Associated with centrosomes and the mitotic spindle during metaphase.
  • Information by UniProt
  • Database links
  • Alternative names
    • Collapsin response mediator protein 1 antibody
    • CRMP 1 antibody
    • CRMP-1 antibody
    • Crmp1 antibody
    • Dihydropyrimidinase like 1 antibody
    • Dihydropyrimidinase related protein 1 antibody
    • Dihydropyrimidinase-related protein 1 antibody
    • DPYL1_HUMAN antibody
    • DPYSL1 antibody
    • DRP 1 antibody
    • DRP-1 antibody
    • DRP1 antibody
    • ULIP-3 antibody
    • Ulip3 antibody
    • Unc-33-like phosphoprotein 3 antibody
    see all

Images

  • Lane 1 : Anti-CRMP1 antibody (ab62558) at 0.5 µg/ml
    Lane 2 : Anti-CRMP1 antibody (ab62558) at 1 µg/ml
    Lane 3 : Anti-CRMP1 antibody (ab62558) at 2 µg/ml

    Lane 1 : 3T3 cell lysate
    Lane 2 : 3T3 cell lysate
    Lane 3 : 3T3 cell lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 62 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
  • ab62558, at 2µg/ml, staining CRMP1 in human brain by immunohistochemistry using paraffin-embedded tissue.
  • Immunofluorescence of CRMP1 in Human Brain cells using ab62558 at 10 ug/ml.

References

This product has been referenced in:
  • Kurnellas MP  et al. Reduced expression of plasma membrane calcium ATPase 2 and collapsin response mediator protein 1 promotes death of spinal cord neurons. Cell Death Differ : (2010). WB, ICC/IF ; Rat . Read more (PubMed: 20489728) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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