Overview

  • Product nameAnti-CRMP1 antibody
    See all CRMP1 primary antibodies
  • Description
    Mouse monoclonal to CRMP1
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment: HVVPEPGSSL LTSFEKWHEA ADTKSCCDYS LHVDITSWYD GVREELEVLV QDKGVNSFQV YMAYKDVYQM SDSQLYEAFT FLKGLGAVIL, corresponding to amino acids 221-310 of human CRMP1 (NP_001014809) with a 26 kDa tag

  • Positive control
    • IMR-32 cell lysate, immunogen

Properties

Applications

Our Abpromise guarantee covers the use of ab76995 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 62 kDa.
ELISA Use at an assay dependent dilution.

Target

  • FunctionNecessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, invasive growth and cell migration.
  • Tissue specificityBrain.
  • Sequence similaritiesBelongs to the DHOase family. Hydantoinase/dihydropyrimidinase subfamily.
  • Cellular localizationCytoplasm. Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle. Associated with centrosomes and the mitotic spindle during metaphase.
  • Information by UniProt
  • Database links
  • Alternative names
    • Collapsin response mediator protein 1 antibody
    • CRMP 1 antibody
    • CRMP-1 antibody
    • Crmp1 antibody
    • Dihydropyrimidinase like 1 antibody
    • Dihydropyrimidinase related protein 1 antibody
    • Dihydropyrimidinase-related protein 1 antibody
    • DPYL1_HUMAN antibody
    • DPYSL1 antibody
    • DRP 1 antibody
    • DRP-1 antibody
    • DRP1 antibody
    • ULIP-3 antibody
    • Ulip3 antibody
    • Unc-33-like phosphoprotein 3 antibody
    see all

Anti-CRMP1 antibody images

  • Anti-CRMP1 antibody (ab76995) at 5 µg/ml + immunogen at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 62 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)
  • Anti-CRMP1 antibody (ab76995) at 5 µg/ml + IMR-32 cell lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 62 kDa
    Observed band size : 62 kDa

References for Anti-CRMP1 antibody (ab76995)

ab76995 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Hippocampus)
Loading amount 5 µg
Specification Hippocampus
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Sep 06 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"