Anti-CRMP1 antibody [EPR14520(B)] - N-terminal (ab180517)
- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-CRMP1 antibody [EPR14520(B)] - N-terminal
See all CRMP1 primary antibodies -
DescriptionRabbit monoclonal [EPR14520(B)] to CRMP1 - N-terminal
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-Pmore details
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Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human CRMP1 aa 50-150 (N terminal). The exact sequence is proprietary.
Database link: Q14194 -
Positive control
- Human cerebellum lysate, Fetal brain lysate, SH-SY5Y cell lysate, Paraffin-embedded Human brain sections.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR14520(B)
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IsotypeIgG
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Research areas
Applications
Our Abpromise guarantee covers the use of ab180517 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 62 kDa. | |
| IHC-P | 1/250 - 1/500. |
Target
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FunctionNecessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, invasive growth and cell migration.
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Tissue specificityBrain.
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Sequence similaritiesBelongs to the DHOase family. Hydantoinase/dihydropyrimidinase subfamily.
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Cellular localizationCytoplasm. Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle. Associated with centrosomes and the mitotic spindle during metaphase.
- Information by UniProt
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Database links
- Entrez Gene: 1400 Human
- Entrez Gene: 12933 Mouse
- Entrez Gene: 25415 Rat
- Omim: 602462 Human
- SwissProt: Q14194 Human
- SwissProt: P97427 Mouse
- SwissProt: Q62950 Rat
- Unigene: 135270 Human
see all -
Alternative names
- Collapsin response mediator protein 1 antibody
- CRMP 1 antibody
- CRMP-1 antibody
see all
Images
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![Western blot - Anti-CRMP1 [EPR14520(B)] antibody - N-terminal (ab180517)](http://a.abcam.com/ps/products/180/ab180517/Images/ab180517-211207-ab180517wb2.jpg)
Western blot - Anti-CRMP1 [EPR14520(B)] antibody - N-terminal (ab180517)All lanes : Anti-CRMP1 antibody [EPR14520(B)] - N-terminal (ab180517) at 1/20000 dilution
Lane 1 : SH-SY5Y lysate
Lane 2 : Fetal brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution
Predicted band size: 62 kDa -
![Western blot - Anti-CRMP1 [EPR14520(B)] antibody - N-terminal (ab180517)](http://a.abcam.com/ps/products/180/ab180517/Images/ab180517-211206-ab180517wb.jpg)
Western blot - Anti-CRMP1 [EPR14520(B)] antibody - N-terminal (ab180517)Anti-CRMP1 antibody [EPR14520(B)] - N-terminal (ab180517) at 1/5000 dilution + Human cerebellum lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution
Predicted band size: 62 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRMP1 [EPR14520(B)] antibody - N-terminal (ab180517)](http://a.abcam.com/ps/products/180/ab180517/Images/ab180517-211208-ab180517IHCp.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRMP1 [EPR14520(B)] antibody - N-terminal (ab180517)Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling CRMP1 with ab180517 at 1/500 dilution. Anti-rabbit HRP seconday antibody was used and counterstaining with Hematoxylin was performed.
References
ab180517 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"