ab62478 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
ab62478, at 1/500 dilution, staining CRMP2 in HeLa cells by Immunofluorescence, in the absence or presence of the immunising peptide.
Western blot - Anti-CRMP2 (phospho T514) antibody (ab62478)
Anti-CRMP2 (phospho T514) antibody (ab62478) at 1 µg/ml + Human brain tissue lysate - total protein (ab29466) at 10 µg
Secondary Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size : 63 kDa Observed band size : 63 kDa Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.
Immunocytochemistry/ Immunofluorescence - Anti-CRMP2 (phospho T514) antibody (ab62478)Image from Ou Y et al., PLoS ONE 7(11): e48773. Fig 5.; doi: 10.1371/journal.pone.0048773. Epub 2012 Nov 21.
Immunofluorescence analysis of RPE cells, staining CRMP2 (phospho T514) with ab62478. Cells were fixed in cold methanol and incubated with primary antibody, followed by an AlexaFluor®488-conjugated anti rabbit IgG.
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