Purification notesab62478 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000.
1/50 - 1/100.
Use a concentration of 1 µg/ml. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).
FunctionNecessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, neuronal growth cone collapse and cell migration.
Sequence similaritiesBelongs to the DHOase family. Hydantoinase/dihydropyrimidinase subfamily.
Post-translational modifications3F4, a monoclonal antibody which strongly stains neurofibrillary tangles in Alzheimer disease brains, specifically labels DPYSL2 when phosphorylated on Ser-518, Ser-522 and Thr-509.
ab62478, at 1/500 dilution, staining CRMP2 in HeLa cells by Immunofluorescence, in the absence or presence of the immunising peptide.
Western blot - Anti-CRMP2 (phospho T514) antibody (ab62478)
Anti-CRMP2 (phospho T514) antibody (ab62478) at 1 µg/ml + Human brain tissue lysate - total protein (ab29466) at 10 µg
Secondary Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size : 63 kDa Observed band size : 63 kDa Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.
Immunocytochemistry/ Immunofluorescence - Anti-CRMP2 (phospho T514) antibody (ab62478)Image from Ou Y et al., PLoS ONE 7(11): e48773. Fig 5.; doi: 10.1371/journal.pone.0048773. Epub 2012 Nov 21.
Immunofluorescence analysis of RPE cells, staining CRMP2 (phospho T514) with ab62478. Cells were fixed in cold methanol and incubated with primary antibody, followed by an AlexaFluor®488-conjugated anti rabbit IgG.
References for Anti-CRMP2 (phospho T514) antibody (ab62478)
This product has been referenced in:
Lim NK et al. Localized changes to glycogen synthase kinase-3 and collapsin response mediator protein-2 in the Huntington's disease affected brain. Hum Mol GenetN/A:N/A (2014).
Read more (PubMed: 24634145) »
Sutinen EM et al. Interleukin-18 alters protein expressions of neurodegenerative diseases-linked proteins in human SH-SY5Y neuron-like cells. Front Cell Neurosci8:214 (2014).
Read more (PubMed: 25147500) »