Anti-CRMP3 antibody (ab36217)
Key features and details
- Rabbit polyclonal to CRMP3
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CRMP3 antibody
See all CRMP3 primary antibodies -
Description
Rabbit polyclonal to CRMP3 -
Host species
Rabbit -
Specificity
No cross reactivity was detected with CRMP1, CRMP2, CRMP4 or CRMP5. -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
A 15 residue synthetic peptide derived from C-terminal domain of human CRMP3.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: Whole serum -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab36217 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000. Predicted molecular weight: 65 kDa.
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IHC-P |
Use at an assay dependent concentration.
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Notes |
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WB
1/2000. Predicted molecular weight: 65 kDa. |
IHC-P
Use at an assay dependent concentration. |
Target
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Function
Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, neuronal growth cone collapse and cell migration. -
Sequence similarities
Belongs to the DHOase family. Hydantoinase/dihydropyrimidinase subfamily. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 10570 Human
- Omim: 608407 Human
- SwissProt: O14531 Human
- Unigene: 100058 Human
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Alternative names
- Collapsin response mediator protein 3 antibody
- CRMP 3 antibody
- CRMP-3 antibody
see all
Images
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All lanes : Anti-CRMP3 antibody (ab36217)
Lane 1 : E.coli transformed with cDNA of human CRMP1
Lane 2 : E.coli transformed with cDNA of human CRMP2
Lane 3 : E.coli transformed with cDNA of human CRMP3
Lane 4 : E.coli transformed with cDNA of human CRMP4
Lane 5 : E.coli transformed with cDNA of human CRMP5
Predicted band size: 65 kDa
Observed band size: 65 kDa -
IHC image of ab36217 staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab36217, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
Datasheets and documents
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Datasheet download
References (2)
ab36217 has been referenced in 2 publications.
- Lin YS et al. Collapsin response mediator protein 5 (CRMP5) causes social deficits and accelerates memory loss in an animal model of Alzheimer's disease. Neuropharmacology 157:107673 (2019). PubMed: 31233825
- Hou ST et al. Collapsin response mediator protein 3 deacetylates histone H4 to mediate nuclear condensation and neuronal death. Sci Rep 3:1350 (2013). ChIP ; Mouse . PubMed: 23443259