Overview

  • Product nameAnti-CRMP3 antibody - ChIP Grade
    See all CRMP3 primary antibodies
  • Description
    Rabbit polyclonal to CRMP3 - ChIP Grade
  • SpecificityNo cross reactivity was detected with CRMP1, CRMP2, CRMP4 or CRMP5.
  • Tested applicationsSuitable for: WB, ChIP, IHC-Fr, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A 15 residue synthetic peptide derived from C-terminal domain of human CRMP3.

Properties

Applications

Our Abpromise guarantee covers the use of ab36217 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 65 kDa.
ChIP Use at an assay dependent concentration. PubMed: 23443259
IHC-Fr 1/500.
IHC-P Use at an assay dependent concentration.

Target

  • FunctionNecessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, neuronal growth cone collapse and cell migration.
  • Sequence similaritiesBelongs to the DHOase family. Hydantoinase/dihydropyrimidinase subfamily.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Collapsin response mediator protein 3 antibody
    • CRMP 3 antibody
    • CRMP-3 antibody
    • CRMP3 antibody
    • Dihydropyrimidinase like 4 antibody
    • Dihydropyrimidinase related protein 4 antibody
    • Dihydropyrimidinase-related protein 4 antibody
    • DPYL4_HUMAN antibody
    • DPYSL4 antibody
    • DRP 4 antibody
    • DRP-4 antibody
    • DRP4 antibody
    • ULIP 4 antibody
    • ULIP-4 antibody
    • ULIP4 antibody
    • UNC33 like phosphoprotein 4 antibody
    • UNC33-like phosphoprotein 4 antibody
    see all

Anti-CRMP3 antibody - ChIP Grade images

  • All lanes : Anti-CRMP3 antibody - ChIP Grade (ab36217)

    Lane 1 : E.coli transformed with cDNA of human CRMP1
    Lane 2 : E.coli transformed with cDNA of human CRMP2
    Lane 3 : E.coli transformed with cDNA of human CRMP3
    Lane 4 : E.coli transformed with cDNA of human CRMP4
    Lane 5 : E.coli transformed with cDNA of human CRMP5


    Predicted band size : 65 kDa
    Observed band size : 65 kDa
  • IHC image of ab36217 staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab36217, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-CRMP3 antibody - ChIP Grade (ab36217)

This product has been referenced in:
  • Hou ST  et al. Collapsin response mediator protein 3 deacetylates histone H4 to mediate nuclear condensation and neuronal death. Sci Rep 3:1350 (2013). ChIP ; Mouse . Read more (PubMed: 23443259) »

See 1 Publication for this product

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