Overview

  • Product nameAnti-CRMP5 antibody
    See all CRMP5 primary antibodies
  • Description
    Mouse monoclonal to CRMP5
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment: SFPDTVYKKL VQREKTLKVR GVDRTPYLGD VAVVVHPGKK EMGTPLADTP TRPVTRHGGM RDLHESSFSL SGSQIDDHVP KRASARILAP PGGRSSGIW, corresponding to amino acids 466-564 of human CRMP5 (NP_064519) with a 26 kDa tag.

  • Positive control
    • CRMP5 transfected 293T cell lysate; recombinant tagged human CRMP5 fragment.

Properties

Applications

Our Abpromise guarantee covers the use of ab77158 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 61 kDa.
ELISA Use at an assay dependent dilution.

Target

Anti-CRMP5 antibody images

  • Anti-CRMP5 antibody (ab77158) at 5 µg/ml + recombinant tagged human CRMP5 fragment (the immunogen) at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 61 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-CRMP5 antibody (ab77158) at 5 µg/ml

    Lane 1 : CRMP5 transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 61 kDa
    Observed band size : 61 kDa

References for Anti-CRMP5 antibody (ab77158)

ab77158 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"