Anti-CRNKL1 antibody [2212C1a] (ab51348)

Overview

  • Product nameAnti-CRNKL1 antibody [2212C1a]
    See all CRNKL1 primary antibodies
  • Description
    Mouse monoclonal [2212C1a] to CRNKL1
  • Tested applicationsSuitable for: ICC/IF, WB, Dot blotmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Recombinant human CRNKL1 fragment

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage bufferPreservative: 0.05% Sodium Azide
    Constituents: 1% BSA, PBS (8mM Sodium phosphate, 3mM Potassium chloride, 140mM Sodium chloride, 1.5mM Potassium phosphate, pH 7.4).
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesAb51348 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG depleted (approximately 95%) fetal bovine serum.
  • ClonalityMonoclonal
  • Clone number2212C1a
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab51348 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50.
WB Use at an assay dependent dilution. Predicted molecular weight: 100 kDa.
Dot blot Use at an assay dependent dilution.

Target

  • FunctionInvolved in pre-mRNA splicing process.
  • Tissue specificityWidely expressed. Highly expressed in testis. Not expressed in brain and lung.
  • Sequence similaritiesBelongs to the crooked-neck family.
    Contains 17 HAT repeats.
  • Cellular localizationNucleus speckle. Cytoplasm. Colocalizes with core spliceosomal snRNP proteins. Also diffusely expressed in cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1200013P10Rik antibody
    • 5730590A01Rik antibody
    • C80326 antibody
    • CLF antibody
    • Clf1 antibody
    • CRN antibody
    • Crn crooked neck like 1 (Drosophila) antibody
    • Crnkl1 antibody
    • CRNL1_HUMAN antibody
    • Crooked neck homolog antibody
    • Crooked neck like protein 1 antibody
    • Crooked neck pre mRNA splicing factor 1 antibody
    • Crooked neck-like protein 1 antibody
    • hCrn antibody
    • MGC55327 antibody
    • MGC93283 antibody
    • MSTP021 antibody
    • SYF3 antibody
    • SYF3 pre mRNA splicing factor antibody
    see all

Anti-CRNKL1 antibody [2212C1a] images

  • Anti-CRNKL1 antibody [2212C1a] (ab51348) + Recombinant CRNKL1

    Predicted band size : 100 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)
  • ab51348 staining CRNKL1 in Human glioblastoma cells by Immunocytochemistry/ Immunofluorescence. Cells were PFA-fixed and permeabilized in 01.% Triton X100 in PBS prior to blocking in 0.5% BSA for 20 minutes at room temperature. The primary antibody was diluted 1/50 in 0.5% BSA in PBS and incubated with the sample for 16 hours at 4°C. The secondary antibody was Alexa Fluor® 488-conjugated Goat anti-Mouse polyclonal, diluted 1/400. Nuclei were counterstained with Hoechst (blue)

    See Abreview

References for Anti-CRNKL1 antibody [2212C1a] (ab51348)

ab51348 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (glioma cell line C6 (rat))
Specification glioma cell line C6 (rat)
Fixative Paraformaldehyde
Permeabilization Yes - 0,1% Triton X 100
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 0.5% · Temperature: RT°C
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Abcam user community

Verified customer

Submitted Sep 23 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (human glioblastoma cell line D54MG)
Specification human glioblastoma cell line D54MG
Fixative Paraformaldehyde
Permeabilization Yes - 0,1% Triton X 100
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 0.5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Sep 21 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (human glioblastoma cell lines)
Specification human glioblastoma cell lines
Fixative Paraformaldehyde
Permeabilization Yes - Tritonx100 0.1% in PBS
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 0.5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Feb 15 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"