Overview

  • Product name
  • Description
    Rabbit polyclonal to CROCC
  • Tested applications
    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: VKRLEKQNLE KDQVNKDLTE KLEALESLRL QEQAALETED GEGLQQTLRD LAQAVLSDSE SGVQLSGSER TADASNGS, corresponding to amino acids 406-483 of Human CROCC

  • Positive control
    • Human fallopian tube tissue; U2OS cells

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121653 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.
ICC/IF Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100

Target

  • Function
    Major structural component of the ciliary rootlet, a cytoskeletal-like structure in ciliated cells which originates from the basal body at the proximal end of a cilium and extends proximally toward the cell nucleus. Contributes to centrosome cohesion before mitosis.
  • Sequence similarities
    Belongs to the rootletin family.
  • Post-translational
    modifications
    Phosphorylated by NEK2 which may regulate its association with centrosomes. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Cytoplasm > cytoskeleton > centrosome > centriole. In ciliated cells, associated with ciliary rootlets. In non-ciliated cells, localized between, around and at the proximal ends of the centrioles. Dissociates from the centrioles at the onset of mitosis and reassociates with them at anaphase.
  • Information by UniProt
  • Database links
  • Alternative names
    • ciliary rootlet coiled coil antibody
    • Ciliary rootlet coiled-coil protein antibody
    • Crocc antibody
    • CROCC_HUMAN antibody
    • KIAA0445 antibody
    • Rootletin antibody
    see all

Images

  • ab121653, at 1/150 dilution, staining CROCC in Human fallopian tube tissue by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue.
  • ab121653 at 4 µg/ml staining CROCC in PFA/Triton X-100 fixed/permeabilized U2OS cells by Immunofluorescence (green).

References

ab121653 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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