Overview

  • Product name
  • Description
    Rabbit polyclonal to CROP
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Chicken, Cow, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human CROP.

    (Peptide available as ab87825.)

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: HeLa; U2OS; Jurkat; HEK293; HepG2; Caco2.

Properties

Applications

Our Abpromise guarantee covers the use of ab79231 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 51 kDa).
ICC/IF Use a concentration of 1 µg/ml.

Target

  • Function
    Binds cAMP regulatory element DNA sequence. May play a role in RNA splicing.
  • Tissue specificity
    Widely expressed. Highest levels in heart, brain, pancreas, thymus, ovary, small intestine and peripheral blood leukocytes, as well as cerebellum, putamen and pituitary gland. Lowest levels in lung, liver and kidney. Also expressed in fetal tissues, including brain, heart, kidney, thymus and lung.
  • Sequence similarities
    Belongs to the Luc7 family.
  • Post-translational
    modifications
    Phosphorylated in vitro by SRPK1, SRPK2 and CLK1.
  • Cellular localization
    Nucleus speckle. The subnuclear localization is affected by cisplatin.
  • Information by UniProt
  • Database links
  • Alternative names
    • cAMP regulatory element-associated protein 1 antibody
    • Cisplatin resistance-associated overexpressed protein antibody
    • Cisplatin resistance-associated-overexpressed protein antibody
    • CRA antibody
    • CRE-associated protein 1 antibody
    • CREAP-1 antibody
    • CREAP1 antibody
    • CROP antibody
    • hLuc7A antibody
    • LC7L3_HUMAN antibody
    • LUC7 like 3 pre mRNA splicing factor antibody
    • Luc7-like protein 3 antibody
    • Luc7A antibody
    • LUC7L3 antibody
    • O48 antibody
    • OA48 18 antibody
    • Okadaic acid-inducible phosphoprotein OA48-18 antibody
    see all

Images

  • All lanes : Anti-CROP antibody (ab79231) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
    Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 6 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 51 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab79231 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab79231, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

ab79231 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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