The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 51 kDa).
Use a concentration of 1 µg/ml.
FunctionBinds cAMP regulatory element DNA sequence. May play a role in RNA splicing.
Tissue specificityWidely expressed. Highest levels in heart, brain, pancreas, thymus, ovary, small intestine and peripheral blood leukocytes, as well as cerebellum, putamen and pituitary gland. Lowest levels in lung, liver and kidney. Also expressed in fetal tissues, including brain, heart, kidney, thymus and lung.
Sequence similaritiesBelongs to the Luc7 family.
Post-translational modificationsPhosphorylated in vitro by SRPK1, SRPK2 and CLK1.
Cellular localizationNucleus speckle. The subnuclear localization is affected by cisplatin.
ICC/IF image of ab79231 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab79231, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-CROP antibody (ab79231)
has not yet been referenced specifically in any publications.
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