Overview

  • Product name
    Anti-CROP antibody
    See all CROP primary antibodies
  • Description
    Rabbit polyclonal to CROP
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Chicken, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Tilapia, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residue 1 and 50 of human CROP (NP_057508.2).

  • Positive control
    • Whole cell lysate from HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab80412 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at 2-5 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.

    • Target

    • Function
      Binds cAMP regulatory element DNA sequence. May play a role in RNA splicing.
    • Tissue specificity
      Widely expressed. Highest levels in heart, brain, pancreas, thymus, ovary, small intestine and peripheral blood leukocytes, as well as cerebellum, putamen and pituitary gland. Lowest levels in lung, liver and kidney. Also expressed in fetal tissues, including brain, heart, kidney, thymus and lung.
    • Sequence similarities
      Belongs to the Luc7 family.
    • Post-translational
      modifications
      Phosphorylated in vitro by SRPK1, SRPK2 and CLK1.
    • Cellular localization
      Nucleus speckle. The subnuclear localization is affected by cisplatin.
    • Information by UniProt
    • Database links
      see all
    • Alternative names
      • cAMP regulatory element-associated protein 1 antibody
      • Cisplatin resistance-associated overexpressed protein antibody
      • Cisplatin resistance-associated-overexpressed protein antibody
      • CRA antibody
      • CRE-associated protein 1 antibody
      • CREAP-1 antibody
      • CREAP1 antibody
      • CROP antibody
      • hLuc7A antibody
      • LC7L3_HUMAN antibody
      • LUC7 like 3 pre mRNA splicing factor antibody
      • Luc7-like protein 3 antibody
      • Luc7A antibody
      • LUC7L3 antibody
      • O48 antibody
      • OA48 18 antibody
      • Okadaic acid-inducible phosphoprotein OA48-18 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CROP antibody (ab80412)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CROP antibody (ab80412)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testicular seminoma (left) and mouse colon carcinoma (right) tissue labelling CROP with ab80412 at 1/1000 (1µg/ml). Detection: DAB.
    • Immunoprecipitation - Anti-CROP antibody (ab80412)
      Immunoprecipitation - Anti-CROP antibody (ab80412)
      Detection of Human CROP in immunoprecipitates of whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab80412 at 3 µg/mg lysate for IP (lane 1). ab80412 was used at 1 µg/ml for subsequent WB detection. Lane 2 represents control IgG IP.

    References

    ab80412 has not yet been referenced specifically in any publications.

    Publishing research using ab80412? Please let us know so that we can cite the reference in this datasheet.

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