The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use at 2-5 µg/mg of lysate.
Is unsuitable for WB.
Binds cAMP regulatory element DNA sequence. May play a role in RNA splicing.
Widely expressed. Highest levels in heart, brain, pancreas, thymus, ovary, small intestine and peripheral blood leukocytes, as well as cerebellum, putamen and pituitary gland. Lowest levels in lung, liver and kidney. Also expressed in fetal tissues, including brain, heart, kidney, thymus and lung.
Belongs to the Luc7 family.
Phosphorylated in vitro by SRPK1, SRPK2 and CLK1.
Nucleus speckle. The subnuclear localization is affected by cisplatin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testicular seminoma (left) and mouse colon carcinoma (right) tissue labelling CROP with ab80412 at 1/1000 (1µg/ml). Detection: DAB.
Detection of Human CROP in immunoprecipitates of whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab80412 at 3 µg/mg lysate for IP (lane 1). ab80412 was used at 1 µg/ml for subsequent WB detection. Lane 2 represents control IgG IP.