Overview

  • Product name
  • Description
    Rabbit polyclonal to CROT
  • Tested applications
    Suitable for: Flow Cyt, WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 57-86 of Human CROT, conjugated to KLH.

  • Positive control
    • WB: K562 cell lysate and mouse liver tissue lysate. IHC-P: Human brain tissue. Flow Cyt: K562 cells. ICC/IF: T24/83 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab103448 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

WB 1/50 - 1/100. Predicted molecular weight: 70 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Function
    Beta-oxidation of fatty acids. The highest activity concerns the C6 to C10 chain length substrate. Converts the end product of pristanic acid beta oxidation, 4,8-dimethylnonanoyl-CoA, to its corresponding carnitine ester.
  • Pathway
    Lipid metabolism; fatty acid beta-oxidation.
  • Sequence similarities
    Belongs to the carnitine/choline acetyltransferase family.
  • Cellular localization
    Peroxisome.
  • Information by UniProt
  • Database links
  • Alternative names
    • Carnitine O octanoyltransferase antibody
    • COT antibody
    • CROT antibody
    • OCTC_HUMAN antibody
    • Peroxisomal carnitine acyltransferase antibody
    • Peroxisomal carnitine O octanoyltransferase antibody
    • Peroxisomal carnitine O-octanoyltransferase antibody
    see all

Images

  • ICC/IF image of ab103448 stained T24/83 cells.  The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum/ 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab103448 at 5 µg/mL overnight at +4°C. The secondary antibody (green) was DyLight® 488 Goat anti-Rabbit IgG (H+L) (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-CROT antibody (ab103448) at 1/50 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : Mouse liver tissue lysate

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 70 kDa
  • ab103448, at a 1/50 dilution, staining CROT in formalin fixed and paraffin embedded Human brain tissue which was peroxidase conjugated to the secondary antibody, followed by DAB staining.
  • Flow cytometric analysis of K562 cells with ab103448 at a 1/10 dilution (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

References

This product has been referenced in:
  • Horie T  et al. MicroRNA-33 deficiency reduces the progression of atherosclerotic plaque in ApoE-/- mice. J Am Heart Assoc 1:e003376 (2012). Mouse . Read more (PubMed: 23316322) »
  • Rayner KJ  et al. Inhibition of miR-33a/b in non-human primates raises plasma HDL and lowers VLDL triglycerides. Nature 478:404-7 (2011). WB . Read more (PubMed: 22012398) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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