Anti-CRSP2 / MED14 antibody (ab196624)
Key features and details
- Rabbit polyclonal to CRSP2 / MED14
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human, African green monkey
- Isotype: IgG
Overview
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Product name
Anti-CRSP2 / MED14 antibody
See all CRSP2 / MED14 primary antibodies -
Description
Rabbit polyclonal to CRSP2 / MED14 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human, African green monkey
Predicted to work with: Mouse -
Immunogen
Synthetic peptide corresponding to Human CRSP2/ MED14 (internal sequence).
Database link: O60244 -
Positive control
- Human brain tissue and COS7 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
(without Mg2+ and Ca2+) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab196624 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/50 - 1/100.
|
|
ICC/IF |
1/100 - 1/500.
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Notes |
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IHC-P
1/50 - 1/100. |
ICC/IF
1/100 - 1/500. |
Target
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Function
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the Mediator complex subunit 14 family. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 9282 Human
- Entrez Gene: 26896 Mouse
- Omim: 300182 Human
- SwissProt: O60244 Human
- SwissProt: A2ABV5 Mouse
- Unigene: 407604 Human
- Unigene: 17616 Mouse
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Alternative names
- Activator-recruited cofactor 150 kDa component antibody
- ARC150 antibody
- Cofactor required for Sp1 transcriptional activation subunit 2 antibody
see all
Images
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Immunofluorscence analysis of COS-7 (African green monkey kidney fibroblast-like cell line) cells labeling CRSP2 / MED14 with ab196624 at a 1/100 dilution.
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Immunohistochemical analysis of formalin fixed and paraffin embedded human brain tissue labeling CRSP2 / MED14 with ab196624 at a 1/50 dilution. Right image treated with synthesized peptide.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab196624 has not yet been referenced specifically in any publications.