Overview

  • Product nameAnti-CRSP2 / MED14 antibody
    See all CRSP2 / MED14 primary antibodies
  • Description
    Rabbit polyclonal to CRSP2 / MED14
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Horse, Dog, Pig, Chimpanzee, Rhesus monkey, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residues 1 and 50 of human CRSP2/ MED14.

  • Positive control
    • Whole cell lysate from Hela cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab72141 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notesIP: Use at 2-5µg/mg of lysate.
    WB: 1/2000 - 1/10000. Detects bands of approximately 71 and 160 kDa and various other bands (predicted molecular weight: 160 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionComponent of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
    • Tissue specificityUbiquitous.
    • Sequence similaritiesBelongs to the Mediator complex subunit 14 family.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Activator-recruited cofactor 150 kDa component antibody
      • ARC150 antibody
      • Cofactor required for Sp1 transcriptional activation subunit 2 antibody
      • CRSP complex subunit 2 antibody
      • DRIP150 antibody
      • EXLM1 antibody
      • hRGR1 antibody
      • MED14 antibody
      • MED14_HUMAN antibody
      • Mediator complex subunit 14 antibody
      • Mediator of RNA polymerase II transcription subunit 14 antibody
      • RGR1 antibody
      • RGR1 homolog antibody
      • Thyroid hormone receptor-associated protein complex 170 kDa component antibody
      • Transcriptional coactivator CRSP150 antibody
      • Trap170 antibody
      • Vitamin D3 receptor-interacting protein complex 150 kDa component antibody
      see all

    Anti-CRSP2 / MED14 antibody images

    • All lanes : Anti-CRSP2 / MED14 antibody (ab72141) at 0.04 µg/ml

      Lane 1 : Whole cell lysate from Hela cells at 50 µg
      Lane 2 : Whole cell lysate from Hela cells at 15 µg
      Lane 3 : Whole cell lysate from Hela cells at 5 µg


      Predicted band size : 160 kDa
      Observed band size : 160 kDa
      Additional bands at : 120 kDa,71 kDa. We are unsure as to the identity of these extra bands.
    • Immunoprecipitation/ Western Blot of CRSP2 / MED14.
      Lane 1: ab72141 at 3µg/mg whole cell lysate for IP.
      Lane 2: Control IgG.

      ab72141 at 1µg/ml for subsequent WB.
      Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
      Detection:Chemiluminescence with an exposure time of 3 seconds.

    References for Anti-CRSP2 / MED14 antibody (ab72141)

    This product has been referenced in:
    • Ruiz A  et al. Characterization of the influence of mediator complex in HIV-1 transcription. J Biol Chem 289:27665-76 (2014). Human . Read more (PubMed: 25100719) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"