Overview

  • Product nameAnti-CRSP7 antibody
    See all CRSP7 primary antibodies
  • Description
    Rabbit polyclonal to CRSP7
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide (Human) derived from the N terminus.

  • Positive control
    • Extracts from LOVO cells.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab65112 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).
ELISA 1/20000.

Target

  • FunctionComponent of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
  • Sequence similaritiesBelongs to the Mediator complex subunit 26 family.
    Contains 1 TFIIS N-terminal domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Activator recruited cofactor 70 kDa component antibody
    • Activator-recruited cofactor 70 kDa component antibody
    • ARC 70 antibody
    • ARC70 antibody
    • Cofactor required for Sp1 transcriptional activation subunit 7 antibody
    • Cofactor required for Sp1 transcriptional activation, subunit 7 (70kD) antibody
    • Cofactor required for Sp1 transcriptional activation, subunit 7, 70kDa antibody
    • CRSP 7 antibody
    • CRSP complex subunit 7 antibody
    • CRSP70 antibody
    • MED 26 antibody
    • Med26 antibody
    • MED26_HUMAN antibody
    • Mediator complex subunit 26 antibody
    • Mediator of RNA polymerase II transcription subunit 26 antibody
    • Transcriptional coactivator CRSP70 antibody
    see all

Anti-CRSP7 antibody images

  • All lanes : Anti-CRSP7 antibody (ab65112) at 1/500 dilution

    Lane 1 : extracts from LOVO cells
    Lane 2 : extracts from LOVO cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 65 kDa
    Observed band size : 65 kDa
    Additional bands at : 100 kDa,34 kDa. We are unsure as to the identity of these extra bands.
  • All lanes : Anti-CRSP7 antibody (ab65112) at 1/1000 dilution

    Lane 1 : Hela Cell Lysate at 20 µg/ml
    Lane 2 : Hela Cell Lysate at 35 µg/ml

    Secondary
    Peroxidase conjugated Goat anti-Rabbit at 1/8000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 65 kDa


    Exposure time : 10 seconds

    This image is courtesy of an anonymous Abreview

    See Abreview

References for Anti-CRSP7 antibody (ab65112)

ab65112 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa Cells)
Loading amount 35 µg
Specification HeLa Cells
Gel Running Conditions Reduced Denaturing (8 % SDS gel)
Blocking step Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Feb 04 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"