Overview

  • Product name
  • Description
    Rabbit polyclonal to CRSP8
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Drosophila melanogaster, Zebrafish
  • Immunogen

    A region within synthetic peptide: VRSFMTWLRS YIKLFQAPCQ RCGKFLQDGL PPTWRDFRTL EAFHDTCRQ, corresponding to C terminal amino acids 263-311 of Human CRSP8

  • Positive control
    • HepG2 cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab66370 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 5 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/62500.

Target

  • Function
    Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
  • Sequence similarities
    Belongs to the Mediator complex subunit 27 family.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cofactor required for Sp1 transcriptional activation subunit 8 (34kD) antibody
    • Cofactor required for Sp1 transcriptional activation subunit 8 34kDa antibody
    • Cofactor required for Sp1 transcriptional activation subunit 8 antibody
    • CRAP 34 antibody
    • CRAP34 antibody
    • CRSP 34 antibody
    • CRSP 8 antibody
    • CRSP complex subunit 8 antibody
    • CRSP34 antibody
    • CRSP8 antibody
    • MED 27 antibody
    • Med27 antibody
    • MED27_HUMAN antibody
    • Mediator complex subunit 27 antibody
    • Mediator of RNA polymerase II transcription subunit 27 antibody
    • MGC11274 antibody
    • P37 TRAP/SMCC/PC2 subunit antibody
    • Transcriptional coactivator CRSP34 antibody
    • TRAP 37 antibody
    • TRAP37 antibody
    see all

Images

  • Anti-CRSP8 antibody (ab66370) at 5 µg/ml + HepG2 cell lysate at 10 µg with 5% skim milk / PBS buffer

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50,000 - 1/100,000 dilution

    Predicted band size : 35 kDa
    Observed band size : 35 kDa

References

ab66370 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab66370.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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