Overview

  • Product name
  • Description
    Rabbit polyclonal to CRSP9
  • Tested applications
    Suitable for: WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CRSP9 aa 90-119 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: O43513

  • Positive control
    • HeLa cells and lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab170390 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 27 kDa.
Flow Cyt 1/10 - 1/50. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
  • Sequence similarities
    Belongs to the Mediator complex subunit 7 family.
  • Post-translational
    modifications
    Constitutively sumoylated.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Activator recruited cofactor 34 kDa component antibody
    • Activator-recruited cofactor 34 kDa component antibody
    • ARC34 antibody
    • Cofactor required for Sp1 transcriptional activation subunit 9 antibody
    • Cofactor required for Sp1 transcriptional activation, subunit 9 (33kD) antibody
    • Cofactor required for Sp1 transcriptional activation, subunit 9, 33kDa antibody
    • CRSP complex subunit 9 antibody
    • CRSP33 antibody
    • CRSP9 antibody
    • hMED7 antibody
    • MED7 antibody
    • MED7_HUMAN antibody
    • Mediator complex subunit 7 antibody
    • Mediator of RNA polymerase II transcription subunit 7 antibody
    • MGC12284 antibody
    • RNA polymerase transcriptional regulation mediator subunit 7 homolog antibody
    • Transcriptional coactivator CRSP33 antibody
    see all

Images

  • Flow cytometric analysis of HeLa cells labeling CRSP9 with ab170390 at 1/10 dilution (right histogram) compared to negative control cells (left histogram). FITC conjugated goat anti-rabbit secondary antibodies were used for the analysis.

  • Anti-CRSP9 antibody (ab170390) at 1/100 dilution + HeLa cell lysate at 35 µg

    Predicted band size : 27 kDa

References

ab170390 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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