1/10 - 1/50. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
Flow cytometric analysis of HeLa cells labeling CRSP9 with ab170390 at 1/10 dilution (right histogram) compared to negative control cells (left histogram). FITC conjugated goat anti-rabbit secondary antibodies were used for the analysis.
Western blot - Anti-CRSP9 antibody (ab170390)
Anti-CRSP9 antibody (ab170390) at 1/100 dilution + HeLa cell lysate at 35 µg