Anti-Cruciform DNA antibody [2D3] (ab150343)

Overview

  • Product name
    Anti-Cruciform DNA antibody [2D3]
  • Description
    Mouse monoclonal [2D3] to Cruciform DNA
  • Tested applications
    Suitable for: WB, EMSAmore details
  • Species reactivity
    Reacts with: a wide range of other species
  • Immunogen

    Heteroduplexes (HindIII-SphI fragments of pRGM21 and pRGM29) mixed (v/v) with 1% methylated bovine serum albumin

Properties

Applications

Our Abpromise guarantee covers the use of ab150343 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.
EMSA Use at an assay dependent concentration. Use 1-10 µg per sample.

Target

  • Relevance
    Inverted repeat sequences are known to form cruciform structures in negatively supercoiled DNA. They are widespread in the genomes of both eukaryotes and prokaryotes, occurring more often than expected among random sequence. In some bacteria, extrusion of cruciform DNA is required for initiation of replication and transcription, though certain inverted repeat sequences are unstable when subcloned into bacteria. Because such structures might impair DNA replication fidelity, the role of inverted repeats in mutagenesis and in human diseases has been widely studied.

References

ab150343 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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