Overview

  • Product nameAnti-CRY2 antibody
    See all CRY2 primary antibodies
  • Description
    Rabbit polyclonal to CRY2
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 543-593 of Human CRY2 (AAH41814.1)

  • Positive control
    • Whole cell lysate from HeLa and 293T cells

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab93802 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notesIP: Use at 2-5µg/mg of lysate.
    WB: 1/2000 - 1/10,000. Predicted molecular weight: 67 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionBlue light-dependent regulator of the circadian feedback loop. Inhibits CLOCK
      NPAS2-ARNTL E box-mediated transcription. Acts, in conjunction with CRY2, in maintaining period length and circadian rhythmicity. Has no photolyase activity. Capable of translocating circadian clock core proteins such as PER proteins to the nucleus. May inhibit CLOCK
      NPAS2-ARNTL transcriptional activity through stabilizing the unphosphorylated form of ARNTL.
    • Tissue specificityExpressed in all tissues examined including fetal brain, fibroblasts, heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon and leukocytes. Highest levels in heart and skeletal muscle.
    • Sequence similaritiesBelongs to the DNA photolyase class-1 family.
      Contains 1 DNA photolyase domain.
    • Post-translational
      modifications
      Phosphorylation on Ser-266 by MAPK is important for the inhibition of CLOCK-ARNTL-mediated transcriptional activity. Phosphorylation by CSKNE requires interaction with PER1 or PER2.
      Ubiquitinated by the SCF(FBXL3) and SCF(FBXL21) complex, leading to its degradation.
    • Cellular localizationCytoplasm. Nucleus. Translocated to the nucleus through interaction with other Clock proteins such as PER2 or ARNTL.
    • Information by UniProt
    • Database links
    • Alternative names
      • cry2 antibody
      • CRY2_HUMAN antibody
      • cryptochrome 2 (photolyase like) antibody
      • Cryptochrome 2 antibody
      • Cryptochrome-2 antibody
      • FLJ10332 antibody
      • growth inhibiting protein 37 antibody
      • HCRY2 antibody
      • KIAA0658 antibody
      • PHLL2 antibody
      • Photolyase like antibody
      see all

    Anti-CRY2 antibody images

    • All lanes : Anti-CRY2 antibody (ab93802) at 0.04 µg/ml

      Lane 1 : whole HeLa cell lysate at 50 µg
      Lane 2 : whole HeLa cell lysate at 15 µg
      Lane 3 : whole HeLa cell lysate at 5 µg
      Lane 4 : whole 293T cell lysate at 50 µg


      Predicted band size : 67 kDa
    • Immunoprecipitation analysis of CRY2 expression in whole HeLa cells lysate (1 mg for IP, 20% of IP loaded) using ab93802 at 3 ug/mg lysate (Left Lane). Right lane represent IP antibody control. Subsequent WB analysis was performed using 1ug/ml ab93802.

    References for Anti-CRY2 antibody (ab93802)

    ab93802 has not yet been referenced specifically in any publications.

    Product Wall

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"