The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at 2-5µg/mg of lysate.
WB: 1/2000 - 1/10,000. Predicted molecular weight: 67 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Blue light-dependent regulator of the circadian feedback loop. Inhibits CLOCK NPAS2-ARNTL E box-mediated transcription. Acts, in conjunction with CRY2, in maintaining period length and circadian rhythmicity. Has no photolyase activity. Capable of translocating circadian clock core proteins such as PER proteins to the nucleus. May inhibit CLOCK NPAS2-ARNTL transcriptional activity through stabilizing the unphosphorylated form of ARNTL.
Expressed in all tissues examined including fetal brain, fibroblasts, heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon and leukocytes. Highest levels in heart and skeletal muscle.
Belongs to the DNA photolyase class-1 family. Contains 1 DNA photolyase domain.
Phosphorylation on Ser-266 by MAPK is important for the inhibition of CLOCK-ARNTL-mediated transcriptional activity. Phosphorylation by CSKNE requires interaction with PER1 or PER2. Ubiquitinated by the SCF(FBXL3) and SCF(FBXL21) complex, leading to its degradation.
Cytoplasm. Nucleus. Translocated to the nucleus through interaction with other Clock proteins such as PER2 or ARNTL.
All lanes : Anti-CRY2 antibody (ab93802) at 0.04 µg/ml
Lane 1 : whole HeLa cell lysate at 50 µg Lane 2 : whole HeLa cell lysate at 15 µg Lane 3 : whole HeLa cell lysate at 5 µg Lane 4 : whole 293T cell lysate at 50 µg
Predicted band size: 67 kDa
Immunoprecipitation - CRY2 antibody (ab93802)
Immunoprecipitation analysis of CRY2 expression in whole HeLa cells lysate (1 mg for IP, 20% of IP loaded) using ab93802 at 3 ug/mg lysate (Left Lane). Right lane represent IP antibody control. Subsequent WB analysis was performed using 1ug/ml ab93802.