Overview

  • Product nameAnti-CRYBB3 antibody
    See all CRYBB3 primary antibodies
  • Description
    Mouse monoclonal to CRYBB3
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity

    Predicted to work with: Human
  • Immunogen

    Recombinant fragment: PHHKLHLFEN PAFSGRKMEI VDDDVPSLWA HGFQDRVASV RAINGTWVGY EFPGYRGRQY VFERGEYRHW NEWDASQPQL QSVRRIRDQK WHKRGRFPSS , corresponding to amino acids 112-212 of Human CRYBB3, with tag (NP_004067)

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • IsotypeIgG2a
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab76642 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notesELISA: Use at an assay dependent dilution.
    WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 24 kDa.
    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionCrystallins are the dominant structural components of the vertebrate eye lens.
    • Involvement in diseaseDefects in CRYBB3 are the cause of cataract congenital nuclear autosomal recessive type 2 (CATCN2) [MIM:609741]. A congenital cataract affecting the central nucleus of the eye. Nuclear cataracts are often not highly visually significant. The density of the opacities varies greatly from fine dots to a dense, white and chalk-like, central cataract. The condition is usually bilateral. Nuclear cataracts are often combined with opacified cortical fibers encircling the nuclear opacity, which are referred to as cortical riders.
    • Sequence similaritiesBelongs to the beta/gamma-crystallin family.
      Contains 4 beta/gamma crystallin 'Greek key' domains.
    • DomainHas a two-domain beta-structure, folded into four very similar Greek key motifs.
    • Information by UniProt
    • Database links
    • Alternative names
      • Beta B3 crystallin antibody
      • Beta crystallin B3 antibody
      • Beta-B3 crystallin antibody
      • Beta-crystallin B3 antibody
      • CRBB3_HUMAN antibody
      • CRYB3 antibody
      • Crybb3 antibody
      see all

    Anti-CRYBB3 antibody images

    • Anti-CRYBB3 antibody (ab76642) at 5 µg/ml + immunogen at 0.2 µg

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

      Predicted band size : 24 kDa
      Observed band size : 36 kDa (why is the actual band size different from the predicted?)

    References for Anti-CRYBB3 antibody (ab76642)

    ab76642 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab76642.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"