Overview

  • Product nameCryptotanshinone
  • Description
    STAT3 inhibitor with diverse biological activities
  • Biological descriptionSTAT3 inhibitor (IC50 = 4.6 μM). Cell-permeable, naturally occuring constitutent of Salvia miltiorrhiza with diverse biological activities. Shows anticancer, antibacterial, anti-inflammatory, antidiabetes and antiobesity activity. Attenuates amyloid plaque deposition in the brain.
  • Purity> 97%

Properties

    Applications

    Our Abpromise guarantee covers the use of ab120666 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Functional Studies Use at an assay dependent concentration.

    Cryptotanshinone images

    • MCF7 cells were incubated at 37°C for 4h with vehicle control (0 µM) and different concentrations of cryptotanshinone (ab120666). Increased expression of JNK1+JNK2 (phospho T183 + Y185) in MCF7cells correlates with an increase in cryptotanshinone concentration, as described in literature.

      Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10 µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab4821 at at 1/1000 dilution and ab8227 at 1 µg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.

    References for Cryptotanshinone (ab120666)

    This product has been referenced in:
    • Shin DS  et al. Cryptotanshinone inhibits constitutive signal transducer and activator of transcription 3 function through blocking the dimerization in DU145 prostate cancer cells. Cancer Res 69:193-202 (2009). Read more (PubMed: 19118003) »
    • Mei Z  et al. Cryptotanshinone, a compound from Salvia miltiorrhiza modulates amyloid precursor protein metabolism and attenuates beta-amyloid deposition through upregulating alpha-secretase in vivo and in vitro. Neurosci Lett 452:90-5 (2009). Read more (PubMed: 19154776) »
    • Kim EJ  et al. Antidiabetes and antiobesity effect of cryptotanshinone via activation of AMP-activated protein kinase. Mol Pharmacol 72:62-72 (2007). Read more (PubMed: 17429005) »

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE, NOT FOR USE IN HUMANS"