• Product nameAnti-CSB antibody [553C5a]
    See all CSB primary antibodies
  • Description
    Mouse monoclonal [553C5a] to CSB
  • Tested applicationsSuitable for: Flow Cyt, WB, Dot Blot, ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to an internal region carrying 50-200 aa of Human CSB


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.05% Sodium Azide
    Constituents: 1% BSA, PBS, 8.0mM Sodium phosphate, 3.0mM Potassium chloride, 140mM Sodium chloride, 1.5mM Potassium phosphate, pH 7.4
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesPurified from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22 µm membrane.
  • ClonalityMonoclonal
  • Clone number553C5a
  • IsotypeIgG2b
  • Research areas


Our Abpromise guarantee covers the use of ab66598 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
WB 1/100. Predicted molecular weight: 168 kDa.
Dot Blot Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. PubMed: 22904069
IP Use at an assay dependent concentration. PubMed: 22904069


  • FunctionEssential factor involved in transcription-coupled nucleotide excision repair which allows RNA polymerase II-blocking lesions to be rapidly removed from the transcribed strand of active genes. Upon DNA-binding, it locally modifies DNA conformation by wrapping the DNA around itself, thereby modifying the interface between stalled RNA polymerase II and DNA. It is required for transcription-coupled repair complex formation. It recruits the CSA complex (DCX(ERCC8) complex), nucleotide excision repair proteins and EP300 to the at sites of RNA polymerase II-blocking lesions.
  • Involvement in diseaseDefects in ERCC6 are the cause of Cockayne syndrome type B (CSB) [MIM:133540]. Cockayne syndrome is a rare disorder characterized by cutaneous sensitivity to sunlight, abnormal and slow growth, cachectic dwarfism, progeroid appearance, progressive pigmentary retinopathy and sensorineural deafness. There is delayed neural development and severe progressive neurologic degeneration resulting in mental retardation. Two clinical forms are recognized: in the classical form or Cockayne syndrome type 1, the symptoms are progressive and typically become apparent within the first few years or life; the less common Cockayne syndrome type 2 is characterized by more severe symptoms that manifest prenatally. Cockayne syndrome shows some overlap with certain forms of xeroderma pigmentosum. Unlike xeroderma pigmentosum, patients with Cockayne syndrome do not manifest increased freckling and other pigmentation abnormalities in the skin and have no significant increase in skin cancer.
    Defects in ERCC6 are the cause of cerebro-oculo-facio-skeletal syndrome type 1 (COFS1) [MIM:214150]; also known as COFS syndrome or Pena-Shokeir syndrome type 2. COFS is a degenerative autosomal recessive disorder of prenatal onset affecting the brain, eye and spinal cord. After birth, it leads to brain atrophy, hypoplasia of the corpus callosum, hypotonia, cataracts, microcornea, optic atrophy, progressive joint contractures and growth failure. Facial dysmorphism is a constant feature. Abnormalities of the skull, eyes, limbs, heart and kidney also occur.
    Defects in ERCC6 are a cause of De Sanctis-Cacchione syndrome (DSC) [MIM:278800]; also known as xerodermic idiocy. DSC is an autosomal recessive syndrome consisting of xeroderma pigmentosum associated with mental retardation, retarded growth, gonadal hypoplasia and sometimes neurologic complications.
    Note=A genetic variation in the 5-prime flanking region of ERCC6 has been shown to be associated with susceptibility to age-related macular degeneration.
    Defects in ERCC6 are a cause of UV-sensitive syndrome (UVS) [MIM:600630]. UVS is a rare autosomal recessive disorder characterized by photosensitivity and mild freckling but without neurological abnormalities or skin tumors.
  • Sequence similaritiesBelongs to the SNF2/RAD54 helicase family.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • DomainA C-terminal ubiquitin-binding domain (UBD) is essential for transcription-coupled nucleotide excision repair to proceed.
  • Post-translational
    Phosphorylated upon DNA damage, probably by ATM or ATR.
    Ubiquitinated at the C-terminus. Ubiquitination by the CSA complex leads to ERCC6 proteasomal degradation in a UV-dependent manner.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 4732403I04 antibody
    • ARMD 5 antibody
    • ARMD5 antibody
    • ATP dependent helicase ERCC6 antibody
    • ATP-dependent helicase ERCC6 antibody
    • C130058G22Rik antibody
    • CKN 2 antibody
    • CKN2 antibody
    • Cockayne syndrome B protein antibody
    • Cockayne syndrome group B protein antibody
    • Cockayne syndrome protein CSB antibody
    • COFS antibody
    • COFS1 antibody
    • CS group B correcting antibody
    • CSB antibody
    • DNA excision repair protein ERCC 6 antibody
    • DNA excision repair protein ERCC-6 antibody
    • ERCC 6 antibody
    • ERCC6 antibody
    • ERCC6_HUMAN antibody
    • Excision repair cross complementing rodent repair deficiency, complementation group 6 antibody
    • OTTHUMP00000019581 antibody
    • RAD26 antibody
    • Rad26 homolog antibody
    • UVSS1 antibody
    see all

Anti-CSB antibody [553C5a] images

  • Overlay histogram showing HeLa cells stained with ab66598 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab66598, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • Anti-CSB antibody [553C5a] (ab66598) + immunising recombinant protein

    Predicted band size : 168 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)
    Primary antibody dilution 1:100, Recombinant protein amount 10ng/lane, Secondary antibody dilution 1:3000, Secondary antibody Sheep anti mouse IgG (0.63mg/ml)

References for Anti-CSB antibody [553C5a] (ab66598)

This product has been referenced in:
  • Batenburg NL  et al. Cockayne Syndrome group B protein interacts with TRF2 and regulates telomere length and stability. Nucleic Acids Res : (2012). ICC/IF, IP ; Human . Read more (PubMed: 22904069) »

See 1 Publication for this product

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