CSEN (Human) 293T Transfected Lysate - (positive control) (ab94275)

Overview

  • Product nameCSEN (Human) 293T Transfected Lysate - (positive control)
  • General notesab94275 is a 293T cell transfected lysate in which Human CSEN has been transiently over-expressed using a pCMV-CSEN plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes
  • Tested applicationsWBmore details

Properties

  • BackgroundFunction: Calcium-dependent transcriptional repressor that binds to the DRE element of genes including PDYN and FOS. Affinity for DNA is reduced upon binding to calcium and enhanced by binding to magnesium. Seems to be involved in nociception. Regulatory subunit of Kv4/D (Shal)-type voltage-gated rapidly inactivating A-type potassium channels. Probably modulates channels density, inactivation kinetics and rate of recovery from inactivation in a calcium-dependent and isoform-specific manner. In vitro, modulates KCND2/Kv4.2 and KCND3/Kv4.3 currents. Involved in KCND2 and probably KCND3 trafficking to the cell surface. May play a role in the regulation of PSEN2 proteolytic processing and apoptosis. Together with PSEN2 involved in modulation of beta-amyloid formation. PTM: Palmitoylated. Palmitoylation enhances association with the plasma membrane. Proteolytically cleaved by caspase-3. Phosphorylation at Ser-63 inhibits cleavage by CASP3. Similarity: Belongs to the recoverin family. Contains 4 EF-hand domains. Tissue specificity: Highly expressed in brain. Widely expressed at lower levels. Expression levels are elevated in brain cortex regions affected by Alzheimer disease.

Applications

Our Abpromise guarantee covers the use of ab94275 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • CSEN (Human) 293T Transfected Lysate - (positive control) images

    • ab94275 at 15µg/lane on an SDS-PAGE gel.
    • All lanes : Anti-CSEN antibody (ab57203) at 1/500 dilution

      Lane 1 : CSEN (Human) 293T Transfected Lysate - (positive control) (ab94275)
      Lane 2 : 293T non-transfected lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

    References for CSEN (Human) 293T Transfected Lysate - (positive control) (ab94275)

    ab94275 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"