The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
This peptide may be used for neutralization and control experiments with the polyclonal antibody that reacts with this product and mouse KChIP3, catalog ab3474. Using a solution of peptide of equal volume and concentration to the corresponding antibody will yield a large molar excess of peptide (70-fold) for competitive inhibition of antibody-protein binding reactions.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
A type potassium channel modulatory protein 3
A-type potassium channel modulatory protein 3
DRE antagonist modulator
EF hand transcription factor
Kv channel interacting protein 3
Kv channel-interacting protein 3
Potassium channel interacting protein 3
FunctionCalcium-dependent transcriptional repressor that binds to the DRE element of genes including PDYN and FOS. Affinity for DNA is reduced upon binding to calcium and enhanced by binding to magnesium. Seems to be involved in nociception. Regulatory subunit of Kv4/D (Shal)-type voltage-gated rapidly inactivating A-type potassium channels, such as KCND2/Kv4.2 and KCND3/Kv4.3. Modulates channel expression at the cell membrane, gating characteristics, inactivation kinetics and rate of recovery from inactivation in a calcium-dependent and isoform-specific manner. May play a role in the regulation of PSEN2 proteolytic processing and apoptosis. Together with PSEN2 involved in modulation of beta-amyloid formation.
Tissue specificityHighly expressed in brain. Widely expressed at lower levels. Expression levels are elevated in brain cortex regions affected by Alzheimer disease.
Sequence similaritiesBelongs to the recoverin family. Contains 4 EF-hand domains.
Post-translational modificationsPalmitoylated. Palmitoylation enhances association with the plasma membrane. Proteolytically cleaved by caspase-3. Phosphorylation at Ser-63 inhibits cleavage by CASP3.
Cellular localizationCytoplasm. Cell membrane. Endoplasmic reticulum. Golgi apparatus. Nucleus. Also membrane-bound, associated with the plasma membrane (PubMed:15485870). In the presence of PSEN2 associated with the endoplasmic reticulum and Golgi. The sumoylated form is present only in the nucleus.