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Rabbit polyclonal to CSK
Predicted to work with:
Mouse, Rat, Horse, Chicken, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide within Human CSK aa 375-425. The exact sequence is proprietary. (NP_004374.1).
Jurkat whole cell lysate.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate pH 7 to 8
Concentration information loading...
Immunogen affinity purified
ab186050 was affinity purified using an epitope specific to CSK immobilized on solid support.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 2-10 µg/mg of lysate.
Is unsuitable for WB.
Specifically phosphorylates 'Tyr-504' on LCK, which acts as a negative regulatory site. Can also act on the LYN and FYN kinases.
Expressed in lung and macrophages.
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSK subfamily.
Contains 1 protein kinase domain. Contains 1 SH2 domain. Contains 1 SH3 domain.
Autophosphorylation of Tyr-304 occurs only at abnormally high CSK concentrations in vitro.
Cytoplasm. Cell membrane. Mainly cytoplasmic, also present in lipid rafts.
Information by UniProt
C SRC antibody
C SRC kinase antibody
C src Tyrosine Kinase antibody
Immunoprecipitation - Anti-CSK antibody (ab186050)
Detection of CSK in immunoprecipitates of Jurkat whole cell lysate (1 mg for IP, 20% of IP loaded) using ab186050 at 6 µg/mg lysate for IP (Lane 1). Lane 2 represents rabbit IgG IP control. An alternative anti-CSK antibody was used at 1 µg/ml for subsequent western blot detection. Detection: Chemiluminescence with an exposure time of 10 seconds.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"