Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Lane 1: Wild-type HAP1 cell lysate (20 µg) Lane 2: CSK knockout HAP1 cell lysate (20 µg) Lane 3: Ramos cell lysate (20 µg) Lane 4: Jurkat cell lysate (20 µg) Lanes 1 - 4: Merged signal (red and green). Green - ab54684 observed at 50 kDa. Red - loading control, ab181602, observed at 37 kDa. ab54684 was shown to recognize CSK when CSK knockout samples were used, along with additional cross-reactive bands. Wild-type and CSK knockout samples were subjected to SDS-PAGE. ab54684 and ab181602 (loading control to GAPDH) were diluted 1 μg/mL and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.
IHC-P - CSK antibody (ab54684)
CSK antibody (ab54684) used in immunohistochemistry at 1ug/ml on formalin fixed and paraffin embedded human colon.
Western blot - CSK antibody (ab54684)
Predicted band size : 51 kDa CSK antibody (ab54684) at 1ug/lane + HL-60 cell lysate at 25ug/lane.