Overview

  • Product nameAnti-CSK antibody
    See all CSK primary antibodies
  • Description
    Mouse monoclonal to CSK
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: MSAIQAAWPS GTECIAKYNF HGTAEQDLPF CKGDVLTIVA VTKDPNWYKA KNKVGREGII PANYVQKREG VKAGTKLSLM PWFHGKITRE QAERLLYPPE , corresponding to amino acids 1-101 of Human CSK

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • IsotypeIgG1
  • Light chain typekappa
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab54684 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 51 kDa.
IHC-P Use a concentration of 1 µg/ml.

Target

  • FunctionSpecifically phosphorylates 'Tyr-504' on LCK, which acts as a negative regulatory site. Can also act on the LYN and FYN kinases.
  • Tissue specificityExpressed in lung and macrophages.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. CSK subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
    Contains 1 SH3 domain.
  • Post-translational
    modifications
    Autophosphorylation of Tyr-304 occurs only at abnormally high CSK concentrations in vitro.
  • Cellular localizationCytoplasm. Cell membrane. Mainly cytoplasmic, also present in lipid rafts.
  • Information by UniProt
  • Database links
  • Alternative names
    • C SRC antibody
    • C SRC kinase antibody
    • C src Tyrosine Kinase antibody
    • C-SRC kinase antibody
    • c-src tyrosine kinase antibody
    • Csk A antibody
    • CSK antibody
    • CSK_HUMAN antibody
    • CYTOPLASMIC TYROSINE KINASE antibody
    • EC 2.7.10.2 antibody
    • MGC112926 antibody
    • MGC117393 antibody
    • MGC154049 antibody
    • P60 Src antibody
    • Protein tyrosine kinase CYL antibody
    • Protein-tyrosine kinase CYL antibody
    • Proto oncogene tyrosine protein kinase antibody
    • Tyrosine protein kinase CSK antibody
    • Tyrosine-protein kinase CSK antibody
    • zgc:154049 antibody
    see all

Anti-CSK antibody images



  • Predicted band size : 51 kDa

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: CSK knockout HAP1 cell lysate (20 µg)
    Lane 3: Ramos cell lysate (20 µg)
    Lane 4: Jurkat cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab54684 observed at 50 kDa. Red - loading control, ab181602, observed at 37 kDa.
    ab54684 was shown to recognize CSK when CSK knockout samples were used, along with additional cross-reactive bands. Wild-type and CSK knockout samples were subjected to SDS-PAGE. ab54684 and ab181602 (loading control to GAPDH) were diluted 1 μg/mL and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

  • CSK antibody (ab54684) used in immunohistochemistry at 1ug/ml on formalin fixed and paraffin embedded human colon.


  • Predicted band size : 51 kDa
    CSK antibody (ab54684) at 1ug/lane + HL-60 cell lysate at 25ug/lane.

References for Anti-CSK antibody (ab54684)

This product has been referenced in:
  • Saud SM  et al. Chemopreventive activity of plant flavonoid isorhamnetin in colorectal cancer is mediated by oncogenic Src and ß-catenin. Cancer Res 73:5473-84 (2013). Read more (PubMed: 23824743) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"