Overview

  • Product name
  • Description
    Mouse monoclonal to CSK
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: MSAIQAAWPS GTECIAKYNF HGTAEQDLPF CKGDVLTIVA VTKDPNWYKA KNKVGREGII PANYVQKREG VKAGTKLSLM PWFHGKITRE QAERLLYPPE , corresponding to amino acids 1-101 of Human CSK

  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Isotype
    IgG1
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab54684 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 51 kDa.
IHC-P Use a concentration of 1 µg/ml.

Target

  • Function
    Specifically phosphorylates 'Tyr-504' on LCK, which acts as a negative regulatory site. Can also act on the LYN and FYN kinases.
  • Tissue specificity
    Expressed in lung and macrophages.
  • Sequence similarities
    Belongs to the protein kinase superfamily. Tyr protein kinase family. CSK subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
    Contains 1 SH3 domain.
  • Post-translational
    modifications
    Autophosphorylation of Tyr-304 occurs only at abnormally high CSK concentrations in vitro.
  • Cellular localization
    Cytoplasm. Cell membrane. Mainly cytoplasmic, also present in lipid rafts.
  • Information by UniProt
  • Database links
  • Alternative names
    • C SRC antibody
    • C SRC kinase antibody
    • C src Tyrosine Kinase antibody
    • C-SRC kinase antibody
    • c-src tyrosine kinase antibody
    • Csk A antibody
    • CSK antibody
    • CSK_HUMAN antibody
    • CYTOPLASMIC TYROSINE KINASE antibody
    • EC 2.7.10.2 antibody
    • MGC112926 antibody
    • MGC117393 antibody
    • MGC154049 antibody
    • P60 Src antibody
    • Protein tyrosine kinase CYL antibody
    • Protein-tyrosine kinase CYL antibody
    • Proto oncogene tyrosine protein kinase antibody
    • Tyrosine protein kinase CSK antibody
    • Tyrosine-protein kinase CSK antibody
    • zgc:154049 antibody
    see all

Anti-CSK antibody images



  • Predicted band size : 51 kDa

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: CSK knockout HAP1 cell lysate (20 µg)
    Lane 3: Ramos cell lysate (20 µg)
    Lane 4: Jurkat cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab54684 observed at 50 kDa. Red - loading control, ab181602, observed at 37 kDa.
    ab54684 was shown to recognize CSK when CSK knockout samples were used, along with additional cross-reactive bands. Wild-type and CSK knockout samples were subjected to SDS-PAGE. ab54684 and ab181602 (loading control to GAPDH) were diluted 1 μg/mL and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

  • CSK antibody (ab54684) used in immunohistochemistry at 1ug/ml on formalin fixed and paraffin embedded human colon.


  • Predicted band size : 51 kDa
    CSK antibody (ab54684) at 1ug/lane + HL-60 cell lysate at 25ug/lane.

References for Anti-CSK antibody (ab54684)

This product has been referenced in:
  • Saud SM  et al. Chemopreventive activity of plant flavonoid isorhamnetin in colorectal cancer is mediated by oncogenic Src and ß-catenin. Cancer Res 73:5473-84 (2013). Read more (PubMed: 23824743) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab54684.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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