Lane 1: Wild-type HAP1 cell lysate (20 µg) Lane 2: CSK knockout HAP1 cell lysate (20 µg) Lane 3: Ramos cell lysate (20 µg) Lane 4: Jurkat cell lysate (20 µg) Lanes 1 - 4: Merged signal (red and green). Green - ab54684 observed at 50 kDa. Red - loading control, ab181602, observed at 37 kDa. ab54684 was shown to recognize CSK when CSK knockout samples were used, along with additional cross-reactive bands. Wild-type and CSK knockout samples were subjected to SDS-PAGE. ab54684 and ab181602 (loading control to GAPDH) were diluted 1 μg/mL and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.
IHC-P - CSK antibody (ab54684)
CSK antibody (ab54684) used in immunohistochemistry at 1ug/ml on formalin fixed and paraffin embedded human colon.
Western blot - CSK antibody (ab54684)
Predicted band size : 51 kDa CSK antibody (ab54684) at 1ug/lane + HL-60 cell lysate at 25ug/lane.