Lane 1: Wild-type HAP1 cell lysate (20 µg) Lane 2: CSK knockout HAP1 cell lysate (20 µg) Lane 3: Ramos cell lysate (20 µg) Lane 4: Jurkat cell lysate (20 µg) Lanes 1 - 4: Merged signal (red and green). Green - ab54684 observed at 50 kDa. Red - loading control, ab181602, observed at 37 kDa. ab54684 was shown to recognize CSK when CSK knockout samples were used, along with additional cross-reactive bands. Wild-type and CSK knockout samples were subjected to SDS-PAGE. ab54684 and ab181602 (loading control to GAPDH) were diluted 1 μg/mL and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
IHC-P - CSK antibody (ab54684)
CSK antibody (ab54684) used in immunohistochemistry at 1ug/ml on formalin fixed and paraffin embedded human colon.
Western blot - CSK antibody (ab54684)
Predicted band size : 51 kDa CSK antibody (ab54684) at 1ug/lane + HL-60 cell lysate at 25ug/lane.
References for Anti-CSK antibody (ab54684)
This product has been referenced in:
Saud SM et al. Chemopreventive activity of plant flavonoid isorhamnetin in colorectal cancer is mediated by oncogenic Src and ß-catenin. Cancer Res73:5473-84 (2013).
Read more (PubMed: 23824743) »