Overview

  • Product name
  • Description
    Goat polyclonal to CSN1
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide: PANSQSRMSTNM, corresponding to C terminal amino acids 480-491 of Human GPS1.

  • General notes
    GenBank Accession Number – NP_004118. Gene Ontology terms - GTPase inhibitor activity; JNK cascade; inactivation of MAPK; cell cycle. LocusLink ID - 2873 (human); 209318 (mouse); 117039 (rat).

Properties

Applications

Our Abpromise guarantee covers the use of ab4535 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 58 kDa).Can be blocked with Human CSN1 peptide (ab23045).

Target

  • Function
    Essential component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. Suppresses G-protein-and mitogen-activated protein kinase-mediated signal transduction.
  • Tissue specificity
    Widely expressed.
  • Sequence similarities
    Belongs to the CSN1 family.
    Contains 1 PCI domain.
  • Domain
    The PCI domain is necessary and sufficient for the interactions with other CSN subunits of the complex. Mediates the interaction with CAPN8.
    The N-terminal part (1-216), which is not required for deneddylating activity and CSN complex formation, is nevertheless essential for other aspects of CSN complex function, such as repression of c-fos/FOS expression.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • COP9 signalosome complex subunit 1 antibody
    • COP9 signalosome subunit 1 antibody
    • COPS1 antibody
    • CSN 1 antibody
    • CSN1_HUMAN antibody
    • FUS6/COP11, Arabidopsis, homolog of antibody
    • G protein pathway suppressor 1 antibody
    • GPS-1 antibody
    • Gps1 antibody
    • JAB1-containing signalosome subunit 1 antibody
    • Protein GPS1 antibody
    • Protein MFH antibody
    • SGN1 antibody
    • Signalosome subunit 1 antibody
    see all

Images



  • Predicted band size : 58 kDa

    ab4535 staining (0.5µg/ml) of Human Testis lysate (RIPA buffer, 30µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence.

    ab4535 staining (0.5µg/ml) of Human Testis lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

References

This product has been referenced in:
  • Lu R  et al. COPS5 amplification and overexpression confers tamoxifen-resistance in ERa-positive breast cancer by degradation of NCoR. Nat Commun 7:12044 (2016). Human . Read more (PubMed: 27375289) »
  • Bauer D  et al. Abnormal expression of glutamate transporter and transporter interacting molecules in prefrontal cortex in elderly patients with schizophrenia. Schizophr Res 104:108-20 (2008). Read more (PubMed: 18678470) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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