Anti-CSN1 (phospho S474) antibody (ab111894)


  • Product name
    Anti-CSN1 (phospho S474) antibody
    See all CSN1 primary antibodies
  • Description
    Rabbit polyclonal to CSN1 (phospho S474)
  • Specificity
    ab111894 detects endogenous levels of CSN1 only when phosphorylated at serine 454.
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic phosphopeptide derived from Human CSN1 around the phosphorylation site of serine 474 (E-G-SP-Q-G).

  • Positive control
    • Human Brain tissue.



Our Abpromise guarantee covers the use of ab111894 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


  • Function
    Essential component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. Suppresses G-protein-and mitogen-activated protein kinase-mediated signal transduction.
  • Tissue specificity
    Widely expressed.
  • Sequence similarities
    Belongs to the CSN1 family.
    Contains 1 PCI domain.
  • Domain
    The PCI domain is necessary and sufficient for the interactions with other CSN subunits of the complex. Mediates the interaction with CAPN8.
    The N-terminal part (1-216), which is not required for deneddylating activity and CSN complex formation, is nevertheless essential for other aspects of CSN complex function, such as repression of c-fos/FOS expression.
  • Post-translational
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • COP9 signalosome complex subunit 1 antibody
    • COP9 signalosome subunit 1 antibody
    • COPS1 antibody
    • CSN 1 antibody
    • CSN1_HUMAN antibody
    • FUS6/COP11, Arabidopsis, homolog of antibody
    • G protein pathway suppressor 1 antibody
    • GPS-1 antibody
    • Gps1 antibody
    • JAB1-containing signalosome subunit 1 antibody
    • Protein GPS1 antibody
    • Protein MFH antibody
    • SGN1 antibody
    • Signalosome subunit 1 antibody
    see all

Anti-CSN1 (phospho S474) antibody images

  • ab111894 at 1/50 dilution staining CSN1 in paraffin-embedded Human Brain tissue by Immunohistochemistry. The image on the right is treated with the synthesized phosphopeptide.

References for Anti-CSN1 (phospho S474) antibody (ab111894)

ab111894 has not yet been referenced specifically in any publications.

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