The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Detects a band of approximately 33 kDa.
FunctionComponent of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, JUN, I-kappa-B-alpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively.
Sequence similaritiesBelongs to the CSN7/EIF3M family. CSN7 subfamily. Contains 1 PCI domain.
RIPA lysate (50µg for E; 25µg for T) from HEK293T cells that were mock transfected (E) or transfected with a CSN7b construct (T). Antibody: ab11895 used at the indicated concentration. Detection: Chemiluminescence with an exposure time of 30 seconds.
References for Anti-CSN7b antibody (ab11895)
This product has been referenced in:
Oh W et al. Jab1 induces the cytoplasmic localization and degradation of p53 in coordination with Hdm2. J Biol Chem281:17457-65 (2006).
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