Overview

  • Product nameAnti-CSRP2 antibody
    See all CSRP2 primary antibodies
  • Description
    Goat polyclonal to CSRP2
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Horse, Cow, Opossum
  • Immunogen

    Synthetic peptide:

    C-ERLGIKPESVQP

    , corresponding to internal sequence amino acids 86-97 of Human CSRP2

  • Positive control
    • Human heart and kidney lysates. Mouse and rat kidney lysates.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab65946 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab65946 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 21 kDa).

Approx 26kDa band observed in Human Heart and Kidney lysates and in Mouse and Rat Kidney lysates (calculated MW of 21.0kDa according to NP_001312.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Huber et al, J Biol Chem. 2000 Feb 25;275(8):5504-11; PMID: 10681529).

IHC-P Use a concentration of 3 - 6 µg/ml.

Target

Anti-CSRP2 antibody images

  • All lanes : Anti-CSRP2 antibody (ab65946) at 0.2 µg/ml

    Lane 1 : Human kidney lysate
    Lane 2 : Mouse kidney lysate
    Lane 3 : Rat kidney lysate

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 21 kDa

References for Anti-CSRP2 antibody (ab65946)

ab65946 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Vascular Smooth Muscle)
Loading amount 20 µg
Specification Vascular Smooth Muscle
Gel Running Conditions Reduced Denaturing (10% Bis-Tris with MOPS running buffer)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Submitted Jul 24 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"