Overview

  • Product nameAnti-CST6 antibody
    See all CST6 primary antibodies
  • Description
    Mouse monoclonal to CST6
  • Tested applicationsSuitable for: WB, IHC-P, IPmore details
  • Species reactivity
    Reacts with: Human, Recombinant Fragment
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 29-150 of Human CST6

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • IsotypeIgG2a
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab54572 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
IHC-P Use at an assay dependent concentration.
IP 1/2500.

Target

  • FunctionShows moderate inhibition of cathepsin B but is not active against cathepsin C.
  • Tissue specificityRestricted to the stratum granulosum of normal skin, the stratum granulosum/spinosum of psoriatic skin, and the secretory coils of eccrine sweat glands. Low expression levels are found in the nasal cavity.
  • Sequence similaritiesBelongs to the cystatin family.
  • Post-translational
    modifications
    Substrate for transglutaminases. Acts as an acyl acceptor but not as an acyl donor.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • CST 6 antibody
    • CST6 antibody
    • Cystatin 6 antibody
    • Cystatin E antibody
    • Cystatin E/M antibody
    • Cystatin M antibody
    • Cystatin M/E antibody
    • Cystatin-6 antibody
    • Cystatin-E antibody
    • Cystatin-M antibody
    • Cystatin6 antibody
    • CystatinE antibody
    • CystatinM antibody
    • Cysteine proteinase inhibitor antibody
    • CYTM_HUMAN antibody
    see all

Anti-CST6 antibody images

  • Western blot against tagged recombinant protein immunogen using ab54572 CST6 antibody at 1ug/ml. Predicted band size of immunogen is 39 kDa.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

  • ab54572 staining CST6 in Human hypertrophic prostate tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 4% serum for 15 minutes at 20°C; antigen retrieval was by heat mediation with citrate buffer (10mM pH6). Samples were incubated with primary antibody (1/100 in 1%BSA + PBS) for 16 hours at 20°C. An undiluted Biotin-conjugated Horse universal polyclonal was used as the secondary antibody.

    See Abreview

References for Anti-CST6 antibody (ab54572)

This product has been referenced in:

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - whole cell (human colon and pancreas)
Total protein in input 5000 µg
Specification human colon and pancreas
Immuno-precipitation step Protein G
Username

Mr. Qing Wang

Verified customer

Submitted Aug 02 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Prostate tissue (hypertrophy))
Specification Prostate tissue (hypertrophy)
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM citrate ph6.0
Permeabilization No
Blocking step Serum as blocking agent for 15 minute(s) · Concentration: 4% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Aug 19 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"