Overview

  • Product name
  • Description
    Goat polyclonal to CST8
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    STNEICAIQENSK

    , corresponding to internal sequence amino acids 100-112 of Human CST8 (NP_005483.1)

  • Positive control
    • HEK293 lysate overexpressing Human CST8 with C-terminal MYC tag

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab106743 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab106743 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 16 kDa.

Target

  • Function
    Performs a specialized role during sperm development and maturation.
  • Tissue specificity
    Proximal caput region of the epididymis. Lower expression in the testis. Within the testis it is localized to the elongating spermatids, whereas within the epididymis it is exclusively synthesized by the proximal caput epithelium.
  • Sequence similarities
    Belongs to the cystatin family.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • CRES antibody
    • CST 8 antibody
    • Cst8 antibody
    • CST8_HUMAN antibody
    • cystatin 8 (cystatin related epididymal specific) antibody
    • Cystatin 8 antibody
    • Cystatin related epididymal specific antibody
    • Cystatin related epididymal specific protein antibody
    • Cystatin related epididymal spermatogenic protein antibody
    • Cystatin related protein epididymis specific antibody
    • Cystatin-8 antibody
    • Cystatin-related epididymal spermatogenic protein antibody
    see all

Images

  • Lane 1 : Anti-CST8 antibody (ab106743) at 1 µg/ml
    Lane 2 : Anti-CST8 antibody (ab106743) at 1 mg/ml
    Lane 3 : MYC tag antibody at 1/1000 dilution

    Lane 1 : HEK293 lysate overexpressing Human CST8 with C-terminal MYC tag
    Lane 2 : HEK293 lysate mock-transfected
    Lane 3 : HEK293 lysate overexpressing Human CST8 with C-terminal MYC tag

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique

    Predicted band size : 16 kDa

References

ab106743 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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