Overview

  • Product name
  • Description
    Rabbit polyclonal to CSTF2T
  • Tested applications
    Suitable for: IHC-P, WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Dog, Pig, Chimpanzee, Gorilla, Soybean, Orangutan, Elephant
  • Immunogen

    Synthetic peptide corresponding to a region between residues 566 and 616 of Human CSTF2T (NP_056050.1).

  • Positive control
    • Whole cell lysate from HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab80275 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 64 kDa).
IP Use at 2-5 µg/mg of lysate.

Target

  • Function
    May play a significant role in AAUAAA-independent mRNA polyadenylation in germ cells. Directly involved in the binding to pre-mRNAs.
  • Sequence similarities
    Contains 1 RRM (RNA recognition motif) domain.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CF 1 64 kDa subunit tau antibody
    • CF-1 64 kDa subunit tau variant antibody
    • CF1 64 kDa subunit, tau variant antibody
    • Cleavage stimulation factor 3' pre RNA, subunit 2 64kDa tau variant antibody
    • Cleavage stimulation factor 64 kDa subunit tau antibody
    • Cleavage stimulation factor 64 kDa subunit tau variant antibody
    • Cleavage stimulation factor 64 kDa subunit, tau variant antibody
    • Cleavage stimulation factor subunit 2 tau antibody
    • Cleavage stimulation factor subunit 2 tau variant antibody
    • CSTF 64 kDa subunit tau antibody
    • CSTF 64 kDa subunit tau variant antibody
    • CSTF 64 kDa subunit, tau variant antibody
    • CstF 64T antibody
    • CSTF2T antibody
    • CSTF2T f antibody
    • CSTFT_HUMAN antibody
    • KIAA0689 antibody
    • TauCstF-64 antibody
    • TauCstF64 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling CSTF2T with ab80275 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-CSTF2T antibody (ab80275) at 0.4 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from HeLa cells at 5 µg


    Predicted band size : 64 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
  • Detection of CSTF2T in Immunoprecipitates of Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab80275 at 3 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent WB detection. Lane 2 represents control IgG IP.

References

ab80275 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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