Overview

  • Product name
  • Description
    Rabbit polyclonal to CSTF3
  • Tested applications
    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Horse, Cow, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residue 1 and 50 of human CSTF3 (NP_001317.1)

  • Positive control
    • Whole cell lysates from HeLa and 293T cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab72299 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Relevance
    CSTF3 (cleavage stimulation factor subunit 3) is required for polyadenylation and 3'-end cleavage of mammalian pre-mRNAs and is part of the CSTF complex. The CSTF complex is composed of CSTF1 (50 kDa subunit), CSTF2 (64 kDa subunit) and CSTF3 (77 kDa subunit). CSTF3 functions as a homodimer and interacts directly with CSTF1 and CSTF2.
  • Cellular localization
    Nuclear
  • Database links
  • Alternative names
    • CF 1 77 kDa subunit antibody
    • Cleavage stimulation factor 3' pre RNA subunit 3 antibody
    • Cleavage stimulation factor 77 kDa subunit antibody
    • Cleavage stimulation factor subunit 3 antibody
    • Cleavage stimulation factor subunit 3 isoform 1 antibody
    • CSTF 3 antibody
    • CSTF 77 antibody
    • CSTF 77 kDa subunit antibody
    • CSTF77 antibody
    • MGC117398 antibody
    • MGC43001 antibody
    • MGC75122 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling CSTF3 with ab72299 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-CSTF3 antibody (ab72299) at 0.04 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from HeLa cells at 5 µg
    Lane 4 : Whole cell lysate from 293T cells at 50 µg


    Predicted band size : 83 kDa
    Observed band size : 83 kDa
    Additional bands at : 150 kDa,31 kDa,50 kDa,60 kDa. We are unsure as to the identity of these extra bands.
  • Detection of Human CSTF3 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab72299 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.

References

ab72299 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab72299.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up