Overview

  • Product nameAnti-CtBP1 antibody
    See all CtBP1 primary antibodies
  • Description
    Rabbit polyclonal to CtBP1
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Chicken, Cat, Dog, Pig
  • Immunogen

    A region within synthetic peptide IPAAVEGIVP SAMSLSHGLP PVAHPPHAPS PGQTVKPEAD RDHASDQL, corresponding to C terminal amino acids 382-429 of Human CtBP1

  • Positive control
    • Jurkat cell lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab49194 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 49 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA 1/62500.

Target

  • FunctionInvolved in controlling the equilibrium between tubular and stacked structures in the Golgi complex. Functions in brown adipose tissue (BAT) differentiation. Corepressor targeting diverse transcription regulators such as GLIS2. Has dehydrogenase activity.
  • Sequence similaritiesBelongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.
  • Post-translational
    modifications
    The level of phosphorylation appears to be regulated during the cell cycle. Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylation by HIPK2 on Ser-422 induces proteasomal degradation.
    ADP-ribosylated; when cells are exposed to brefeldin A.
    Sumoylation on Lys-428 is promoted by the E3 SUMO-protein ligase CBX4.
  • Cellular localizationCytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • BARS antibody
    • brefeldin A- ribosylated substrate antibody
    • C terminal binding protein 1 antibody
    • C-terminal-binding protein 1 antibody
    • CTBP antibody
    • CtBP1 antibody
    • CTBP1_HUMAN antibody
    • MGC104684 antibody
    see all

Anti-CtBP1 antibody images

  • Anti-CtBP1 antibody (ab49194) at 1.25 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 49 kDa
    Observed band size : 47 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry of Human Pancreas lysate tissue at an antibody concentration of 4-8µg/ml using anti-CTBP1 antibody (ab49194)

References for Anti-CtBP1 antibody (ab49194)

ab49194 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"